Crystallization (Comment) | Organism |
---|---|
purified enzyme, hangingdrop vapour-diffusion method, 4°C, 11.5 mg/ml protein, the mother liquor contains 32% PEG 200, 0.15 M MES, pH 5.8-6.2, soaking of the crystals in the dark for 12 h at 4°C in mother liquor containing 5 mM [1-(2-nitrophenyl)-2,2,2-trifluoroethyl]-arsenocholine iodide, pH 6.0, X-ray diffraction structure determination and anaylsis at 2.4 A resolution | Tetronarce californica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
[1-(2-nitrophenyl)-2,2,2-trifluoroethyl]-arsenocholine iodide | caged compound, binding structure and mechanism with AChE, binding within the active-site gorge, overview | Tetronarce californica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tetronarce californica | P04058 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylthiocholine + H2O | catalytic residues are Ser200 and Trp84 | Tetronarce californica | thiocholine + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | two domains on either side of the active-site gorge, residues 4-305 and residues 306-535, the catalytic Ser200 and Trp84 are shown at the bottom of the gorge and the peripheral-site Trp279 at its rim, three-dimensional structure, overview | Tetronarce californica |
Synonyms | Comment | Organism |
---|---|---|
AChE | - |
Tetronarce californica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Tetronarce californica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Tetronarce californica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
[1-(2-nitrophenyl)-2,2,2-trifluoroethyl]-arsenocholine iodide | pH 7.0, 20°C | Tetronarce californica |