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Literature summary for 3.1.1.7 extracted from

  • Kato, Y.; Tanaka, T.; Miyata, T.
    Comparison of kinetic properties of a hydrophilic form of acetylcholinesterase purified from strains susceptible and resistant to carbamate and organophosphorus insecticides of green rice leafhopper (Nephotettix cincticeps Uhler) (2004), Pestic. Biochem. Physiol., 79, 64-73.
No PubMed abstract available
Search for more literature for 3.1.1.7

Inhibitors

Inhibitors Comment Organism Structure
acetylthiocholine N-methyl carbamate-resistant strain, no substrate inhibition up to 0.01 mM Nephotettix cincticeps Uhler
butyrylthiocholine N-methyl carbamate-resistant strain, no substrate inhibition up to 0.01 mM Nephotettix cincticeps Uhler
Monocrotophos N-methyl carbamate-resistant strain is 65fold more sensitive than susceptible strain Nephotettix cincticeps Uhler
propionylthiocholine N-methyl carbamate-resistant strain, no substrate inhibition up to 0.01 mM Nephotettix cincticeps Uhler
propoxur N-methyl carbamate-resistant strain is 53fold less sensitive than susceptible strain Nephotettix cincticeps Uhler

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0391
-
 propionylthiocholine pH 7.5, 25°C, N-methyl carbamate-susceptible strain Nephotettix cincticeps Uhler
0.0461
-
 butyrylthiocholine pH 7.5, 25°C, N-methyl carbamate-susceptible strain Nephotettix cincticeps Uhler
0.0511
-
 acetylthiocholine pH 7.5, 25°C, N-methyl carbamate-susceptible strain Nephotettix cincticeps Uhler
0.0852
-
 butyrylthiocholine pH 7.5, 25°C, N-methyl carbamate-resisitant strain Nephotettix cincticeps Uhler
0.0881
-
 propionylthiocholine pH 7.5, 25°C, N-methyl carbamate-resistant strain Nephotettix cincticeps Uhler
0.0918
-
 acetylthiocholine pH 7.5, 25°C, N-methyl carbamate-resistant strain Nephotettix cincticeps Uhler

Organism

Organism UniProt Comment Textmining
Nephotettix cincticeps Uhler
-
 strains susceptible and resistant to carbamate and organophosphorus insecticides
-

Purification (Commentary)

Purification (Comment) Organism
-
 Nephotettix cincticeps Uhler

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6
-
 pH 7.5, 25°C, substrate butyrylthiocholine, N-methyl carbamate-susceptible strain Nephotettix cincticeps Uhler
14.5
-
 pH 7.5, 25°C, substrate butyrylthiocholine, N-methyl carbamate-resisitant strain Nephotettix cincticeps Uhler
27
-
 pH 7.5, 25°C, substrate propionylthiocholine, N-methyl carbamate-resistant strain Nephotettix cincticeps Uhler
30.5
-
 pH 7.5, 25°C, substrate propionylthiocholine, N-methyl carbamate-susceptible strain Nephotettix cincticeps Uhler
70.2
-
 pH 7.5, 25°C, substrate acetylthiocholine, N-methyl carbamate-susceptible strain Nephotettix cincticeps Uhler
123
-
 pH 7.5, 25°C, substrate acetylthiocholine, N-methyl carbamate-resistant strain Nephotettix cincticeps Uhler

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylthiocholine + H2O
-
 Nephotettix cincticeps Uhler thiocholine + acetate
-
 ?
butyrylthiocholine + H2O
-
 Nephotettix cincticeps Uhler butyrate + thiocholine
-
 ?
propionylthiocholine + H2O
-
 Nephotettix cincticeps Uhler propanoate + thiocholine
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 both isoform isolated from strain susceptible and from strain resistant to carbamate and organophosphorus insecticides Nephotettix cincticeps Uhler