Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.61 extracted from

  • Anand, G.S.; Goudreau, P.N.; Stock, A.M.
    Activation of methylesterase CheB: evidence of a dual role for the regulatory domain (1998), Biochemistry, 37, 14038-14047.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

Protein Variants Comment Organism
A137T 3fold higher enzyme activity than wild-type enzyme in absence of phosphoramidate, in presence of phosphoramidate mutant and wild-type proteins show similar increase in enzyme activity Salmonella enterica subsp. enterica serovar Typhimurium
D56N no activation of enzyme activity in the presence of phosphoramidate Salmonella enterica subsp. enterica serovar Typhimurium
H138R 2.5fold higher enzyme activity than wild-type enzyme in absence of phosphoramidate, in presence of phosphoramidate mutant and wild-type proteins show similar increase in enzyme activity Salmonella enterica subsp. enterica serovar Typhimurium
L153P 2fold higher enzyme activity than wild-type enzyme in absence of phosphoramidate, in presence of phosphoramidate mutant and wild-type proteins show similar increase in enzyme activity Salmonella enterica subsp. enterica serovar Typhimurium
R255W 4fold higher enzyme activity than wild-type enzyme in absence of phosphoramidate, in presence of phosphoramidate mutant and wild-type proteins show similar increase in enzyme activity Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ phosphorylation of intact enzyme requires Mg2+ Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Salmonella enterica subsp. enterica serovar Typhimurium demethylation of chemotactic membrane receptors ?
-
?

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein phosphorylation by phosphoramidate requires Mg2+, phosphorylation of the regulatory domain at Asp-56 results in a reorganization of the domain interface, allowing exposure of the active site to the receptor substrate and simultaneously stimulating methylesterase activity Salmonella enterica subsp. enterica serovar Typhimurium

Reaction

Reaction Comment Organism Reaction ID
protein L-glutamate O5-methyl ester + H2O = protein L-glutamate + methanol mechanism of regulation of enzyme activity by the N-terminal domain Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information demethylation of chemotactic membrane receptors Salmonella enterica subsp. enterica serovar Typhimurium ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Salmonella enterica subsp. enterica serovar Typhimurium