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Literature summary for 3.1.1.61 extracted from
- Carboxylmethyl esterase of bacterial chemotaxis (1984), Methods Enzymol., 106, 321-330.
General Stability
| General Stability | Organism |
|---|---|
| maximal stability and low activity is observed in phosphate buffer at pH 7 with 50-100 mM added NaCl, while MES and low salt concentration afford minimum protection against inactivation | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,4,6-Trinitrobenzene sulfonate | - |
Salmonella enterica subsp. enterica serovar Typhimurium |  |
| 2-Hydroxy-5-nitrobenzyl bromide | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| 5,5'-dithiobis(2-nitrobenzoate) | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| diethyl dicarbonate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| iodoacetamide | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| iodoacetate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| methyl methanothiosulfonate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| N-Acetylimidazole | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| NEM | slight | Salmonella enterica subsp. enterica serovar Typhimurium | |
| Phenylglyoxal | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
methyl-accepting chemotaxis protein | pH 7.0, 30°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.026 | - |
methylated prolactin | pH 7.0, 30°C | Salmonella enterica subsp. enterica serovar Typhimurium |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | as effective as Mg2+ in activation | Salmonella enterica subsp. enterica serovar Typhimurium | |
| Mg2+ | divalent cation required | Salmonella enterica subsp. enterica serovar Typhimurium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
x * 37000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Salmonella enterica subsp. enterica serovar Typhimurium | responsible for the demethylation of the methyl-accepting chemotaxis proteins | ? | - |
? | |
| additional information | Salmonella enterica subsp. enterica serovar Typhimurium ST89pGK2 | responsible for the demethylation of the methyl-accepting chemotaxis proteins | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
| Salmonella enterica subsp. enterica serovar Typhimurium ST89pGK2 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| strain ST89pGK2, plasmid bearing strain overproducing esterase and transferase | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 10% glycerol, 0.04% sodium azide, stable for at least several months | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methyl-accepting chemotaxis protein + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | chemotaxis protein + methanol | - |
? | |
| methyl-accepting chemotaxis protein + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium ST89pGK2 | chemotaxis protein + methanol | - |
? | |
| methylated prolactin + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | prolactin + methanol | - |
? | |
| methylated prolactin + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium ST89pGK2 | prolactin + methanol | - |
? | |
| additional information | responsible for the demethylation of the methyl-accepting chemotaxis proteins | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| additional information | responsible for the demethylation of the methyl-accepting chemotaxis proteins | Salmonella enterica subsp. enterica serovar Typhimurium ST89pGK2 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
methyl-accepting chemotaxis protein | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
9.3 |
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