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Literature summary for 3.1.1.5 extracted from

  • De Jong, J.G.N.; van den Bosch, H.; Rijken, D.; van Deenen, L.L.M.
    Studies on lysophospholipases. III. The complete purification of two proteins with lysophospholipase activity from beef liver (1974), Biochim. Biophys. Acta, 369, 50-63.
    View publication on PubMed
Search for more literature for 3.1.1.5

Inhibitors

Inhibitors Comment Organism Structure
bis(p-nitrophenyl)phosphate 0.01 mM, 8% inhibition of lysophospholipase I and 61% inhibition of lysophospholipase II Bos taurus
deoxycholate
-
 Bos taurus
Dithionitrobenzoate
-
 Bos taurus
iodoacetamide
-
 Bos taurus
NEM
-
 Bos taurus
p-hydroxymercuribenzoate
-
 Bos taurus
Triton X-100
-
 Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 x * 24000, lysophospholipase I, SDS-PAGE Bos taurus
26000
-
 lysophospholipase I, SDS-PAGE Bos taurus
57000
-
 lysophospholipase II, SDS-PAGE Bos taurus
63000
-
 x * 63000, lysophospholipase II, SDS-PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 lysophospholipase I and lysophospholipase II
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lysophosphatidylcholine + H2O
-
 Bos taurus glycerophosphorylcholine + unesterified fatty acid
-
 ?
p-nitrophenyl acetate + H2O
-
 Bos taurus p-nitrophenol + acetate
-
 ?
tributyrin + H2O
-
 Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 63000, lysophospholipase II, SDS-PAGE Bos taurus
? x * 24000, lysophospholipase I, SDS-PAGE Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8 deacylation of 2-acyllysophosphatidylcholine, lysophospholipase I Bos taurus
8.5
-
 deacylation of 2-acyllysophosphatidylcholine, lysophospholipase II Bos taurus