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Literature summary for 3.1.1.5 extracted from
- Characterization of the Vibrio cholerae VolA surface-exposed lipoprotein lysophospholipase (2014), J. Bacteriol., 196, 1619-1626.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EGTA | EGTA by itself or in combination with EDTA causes a slight increase in activity | Vibrio cholerae serotype O1 |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21Star (DE3) cells | Vibrio cholerae serotype O1 |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S551A | inactive | Vibrio cholerae serotype O1 |
| S551T | inactive | Vibrio cholerae serotype O1 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the addition of EDTA alone had no effect on the lipase activity | Vibrio cholerae serotype O1 |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell surface | - |
Vibrio cholerae serotype O1 | 9986 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | VolA is a metal-independent enzyme | Vibrio cholerae serotype O1 |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 81600 | - |
x * 81600, SDS-PAGE | Vibrio cholerae serotype O1 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-lysophosphatidylcholine + H2O | Vibrio cholerae serotype O1 | - |
glycerophosphocholine + a carboxylate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio cholerae serotype O1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-Sepharose 6 Fast Flow column chromatography and Superdex 200 gel filtration | Vibrio cholerae serotype O1 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.404 | - |
purified enzyme, at pH 8.0 and 37°C | Vibrio cholerae serotype O1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-palmitoyl-sn-glycero-3-phosphoethanolamine + H2O | - |
Vibrio cholerae serotype O1 | palmitic acid + glycero-3-phosphoethanolamine | - |
? | |
| 2-lysophosphatidylcholine + H2O | - |
Vibrio cholerae serotype O1 | glycerophosphocholine + a carboxylate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 81600, SDS-PAGE | Vibrio cholerae serotype O1 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| VolA | - |
Vibrio cholerae serotype O1 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Vibrio cholerae serotype O1 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Vibrio cholerae serotype O1 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the lipase activity of surface-exposed VolA contributes to the success of Vibrio cholerae, improving the overall adaptation and survival of the organism in different environments | Vibrio cholerae serotype O1 |
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