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Literature summary for 3.1.1.5 extracted from

  • Wright, L.C.; Payne, J.; Santangelo, R.T.; Simpanya, M.F.; Chen, S.C.; Widmer, F.; Sorrell, T.C.
    Cryptococcal phospholipases: a novel lysophospholipase discovered in the pathogenic fungus Cryptococcus gattii (2004), Biochem. J., 384, 377-384.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.5

Activating Compound

Activating Compound Comment Organism Structure
carnitine 10 mM, activation to 121% of control at pH 5.0, LPL1 Cryptococcus gattii VGIII
dithiothreitol 10 mM, activation to 122% of control at pH 5.0, LPL1 Cryptococcus gattii VGIII
EGTA 10 mM, 11% activation of phospholipase B activity Cryptococcus gattii VGIII
lysophosphatidylcholine 0.5 mM, activation of phospholipase B activity to 132% of control Cryptococcus gattii VGIII
palmitoyl carnitine 0.5 mM, stimulates to 157% of control at pH 7.0, LPL1 Cryptococcus gattii VGIII
palmitoyl carnitine 1 mM, activation of lysophospholipase activity at pH 7.0 to 239% of control Cryptococcus gattii VGIII

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 10 mM, 22% inhibition of phospholipase B activity, slight activation of lysophospholipase activity Cryptococcus gattii VGIII
carnitine 10 mM, 38% inhibition of phospholipase B activity; 10 mM, 9% inhibition at pH 7.0, LPL1 Cryptococcus gattii VGIII
dipalmitoyl phosphatidylcholine 0.5 mM, 84% inhibition at pH 5.0, 50% inhibition at pH 7.0, LPL 1 Cryptococcus gattii VGIII
dipalmitoylphosphatidylcholine 0.5 mM, 89% inhibition of lysophospholipase at pH 5.0, 82% inhibition of lysophospholipase at pH 7.0 Cryptococcus gattii VGIII
dithiothreitol 10 mM, 9% inhibition at pH 7.0, LPL1 Cryptococcus gattii VGIII
EGTA 10 mM, 15% inhibition at pH 7.0, LPL1 Cryptococcus gattii VGIII
FeCl3 10 mM, 39% inhibition at pH 5.0, 38% inhibition at pH 7.0, LPL1; 10 mM, 80% inhibiotion of phospholipase B activity, 17% inhibition of lysophospholipase activity at pH 5.0, 29% in inhibition of lysophospholipase activity at pH 7.0 Cryptococcus gattii VGIII
palmitoyl carnitine 0.5 mM, 90% inhibition at pH 5.0, LPL1; 1 mM, 64% inhibition of phospholipase B activity, 65% inhibition of lysophospholipase activity at pH 5.0 Cryptococcus gattii VGIII
phosphatidic acid 0.5 mM, 24% inhibition at pH 5.0, 66% inhibition at pH 7.0, LPL1; 0.5 mM, 58% inhibition of phospholipase B activity, 92% inhibition of lysophospholipase at pH 5.0, 87% inhibition of lysophospholipase at pH 7.0 Cryptococcus gattii VGIII
sphingomyelin 0.5 MM, 21% inhibition at pH 5.0, 29% inhibition at pH 7.0, LPL1; 0.5 mM, 35% inhibition of phospholipase B activity, 45% inhibition of lysophospholipase at pH 5.0, 28% inhibition of lysophospholipase at pH 7.0 Cryptococcus gattii VGIII
Triton X-100 0.1 w/v, 48% inhibition at pH 5.0, LPL1; 0.1% w/v, 85% inhibition of phospholipase B activity, 91% inhibition of lysophospholipase B activity at pH 5.0, 95% inhibition of lysophospholipase B activity at pH 7.0 Cryptococcus gattii VGIII

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 10 mM, stimulates to 260% of control at pH 7.0, but not at pH 5.0, LPL1 Cryptococcus gattii VGIII
CaCl2 10 mM, 22% inhibition of phospholipase B activity, slight activation of lysophospholipase activity, to 8% at pH 5.0 and 4% at pH 7.0 Cryptococcus gattii VGIII

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 10 * 66000, isoenzyme LPL1, SDS-PAGE Cryptococcus gattii VGIII
220000
-
 isoenzyme LPL1, gel filtratiin Cryptococcus neoformans
275000
-
 isoenzyme PLB1, gel filtratiin Cryptococcus gattii VGIII
670000
-
-
 Cryptococcus gattii VGIII

Organism

Organism UniProt Comment Textmining
Cryptococcus gattii VGIII
-
-
-
Cryptococcus neoformans
-
 var. grubii, strain H99
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Cryptococcus gattii VGIII

Purification (Commentary)

Purification (Comment) Organism
isoenzyme LPL1 Cryptococcus neoformans
isoenzyme PLB1 Cryptococcus gattii VGIII

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-oleoyl lysophosphatidylcholine + H2O 30% of the activity with 1-palmitoyl lysophosphatidylcholine, LPL1 Cryptococcus gattii VGIII glycerophosphocholine + oleate
-
 ?
1-oleoyl-2-lysophosphatidylcholine + H2O 30% of the activity with 1-palmitoyl lysophosphatidylcholine at pH 5.0, 667% of the activity with 1-palmitoyl lysophosphatidylcholine at pH 7.0 Cryptococcus gattii VGIII glycerophosphorylcholine + oleic acid
-
 ?
1-palmitoyl lysophosphatidylcholine + H2O preferred substrate, LPL1 Cryptococcus gattii VGIII glycerophosphocholine + palmitate
-
 ?
1-palmitoyl lysophosphatidylcholine + H2O
-
 Cryptococcus gattii VGIII glycerophosphocholine + palmitic acid
-
 ?
1-palmitoyl-lysophosphatidylethanolamine + H2O as active as 1-palmitoyl lysophosphatidylcholine at pH 7.0 Cryptococcus gattii VGIII glycerophosphatidylethanolamine + palmitic acid
-
 ?
1-palmitoyl-lysophosphatidylethanolamine + H2O 20% of the activity with 1-palmitoyl lysophosphatidylcholine, LPL1 Cryptococcus gattii VGIII glycerophosphoethanolamine + palmitate
-
 ?
1-palmitoyl-sn-glycero-3-phosphoserine + H2O as active as 1-palmitoyl lysophosphatidylcholine at pH 7.0, 70% of the activity with 1-palmitoyl lysophosphatidylcholine at pH 5.0 Cryptococcus gattii VGIII glycerophosphoserine + palmitic acid
-
 ?
dioleoylphosphatidylcholine + H2O 2% of the activity with dipalmitoylphosphatidylcholine Cryptococcus gattii VGIII ?
-
 ?
dioleoylphosphatidylethanolamine + H2O 11% of the activity with dipalmitoylphosphatidylcholine Cryptococcus gattii VGIII ?
-
 ?
dipalmitoylphosphatidylcholine + H2O
-
 Cryptococcus gattii VGIII ?
-
 ?
additional information PLB1 shows phospholipase B activity, lysophospholipase activity and transacylase activity Cryptococcus gattii VGIII ?
-
 ?
additional information the LPL1 protein only possesses lysophospholipase and transacylase activity Cryptococcus neoformans ?
-
 ?
additional information the LPL1 protein only possesses lysophospholipase and transacylase activity Cryptococcus gattii VGIII ?
-
 ?
sphingomyelin + H2O 3% of the activity with dipalmitoylphosphatidylcholine Cryptococcus gattii VGIII ?
-
 ?

Subunits

Subunits Comment Organism
decamer 10 * 66000, isoenzyme LPL1, SDS-PAGE Cryptococcus gattii VGIII

Synonyms

Synonyms Comment Organism
LPL1
-
 Cryptococcus neoformans
LPL1
-
 Cryptococcus gattii VGIII
PLB1 enzyme
-
 Cryptococcus gattii VGIII

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Cryptococcus gattii VGIII
37
-
 phospholipase B activity, lysophospholipase activity and transacylase activity of PLB1, no activity detectable at higher temperatures Cryptococcus gattii VGIII

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5 phospholipase B activity, lysophospholipase activity and transacylase activity of PLB1 Cryptococcus gattii VGIII
5
-
 lysophospholipase activity and transacylase activity, short incubation time (30 s), LPL1 Cryptococcus gattii VGIII
5.5 8 lysophospholipase activity, long incubation time (5 min), LPL1 Cryptococcus gattii VGIII

pH Range

pH Minimum pH Maximum Comment Organism
4.2 6 pH 4.2: about 75% of maximal lysophospholipase activity, pH 6.0: about 75% of maximal lysophospholipase activity, short incubation time (30 s), LPL1 Cryptococcus gattii VGIII
7
-
 no phospholipase B activity, no lysophospholipase activity and no transacylase activity of PLB1 is detected at short incubation time (30 s), at incubation time 5-10 min lysophospholipase activity and transacylase activity is detected (about 5% of the activity at pH 5.0), no phospholipase B activity is detected at incubation time 5-10 min Cryptococcus gattii VGIII

pI Value

Organism Comment pI Value Maximum pI Value
Cryptococcus gattii VGIII isoelectric focusing
-
 3.9
Cryptococcus gattii VGIII isoelectric focusing, isoenzyme LPL1
-
 6.3