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Literature summary for 3.1.1.32 extracted from
- Purification and properties of a membrane-bound phospholipase A1 from Mycobacterium phlei (1974), J. Biol. Chem., 249, 5658-5667.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | increases activity | Mycolicibacterium phlei |  |
| Triton X-100 | 0.05% w/v causes maximal enhancement of activity | Mycolicibacterium phlei | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10 mM, 20-60% increase in activity | Mycolicibacterium phlei | |
| EDTA | 10 mM, 30-40% inhibition | Mycolicibacterium phlei | |
| Fe2+ | 5 mM | Mycolicibacterium phlei | |
| Fe3+ | 1 mM, almost complete inhibition | Mycolicibacterium phlei | |
| Hg2+ | 5 mM | Mycolicibacterium phlei | |
| lauryldimethylbenzyl ammonium chloride | - |
Mycolicibacterium phlei | |
| SDS | - |
Mycolicibacterium phlei | |
| sodium deoxycholate | - |
Mycolicibacterium phlei | |
| Triton X-100 | at high concentration | Mycolicibacterium phlei | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | tightly bound | Mycolicibacterium phlei | 5886 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycolicibacterium phlei | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mycolicibacterium phlei |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Mycolicibacterium phlei |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for at least 1 year | Mycolicibacterium phlei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cardiolipin + H2O | - |
Mycolicibacterium phlei | ? | - |
? | |
| phosphatidylcholine + H2O | - |
Mycolicibacterium phlei | ? | - |
? | |
| phosphatidylethanolamine + H2O | - |
Mycolicibacterium phlei | ? | - |
? | |
| phosphatidylglycerol + H2O | - |
Mycolicibacterium phlei | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.7 | - |
hydrolysis of cardiolipin | Mycolicibacterium phlei |
| 4.5 | - |
hydrolysis of phosphatidylglycerol | Mycolicibacterium phlei |
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