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Literature summary for 3.1.1.32 extracted from
- Phosphatidic acid (PA)-preferring phospholipase A1 regulates mitochondrial dynamics (2014), J. Biol. Chem., 289, 11497-11511.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of FLAG-tagged wild-type and mutant enzymes in HeLa cells and in siRNA-treated knockout mutant HeLa cells. Upon overexpression of wild-type enzyme, the tubular structures become fragmented, expression of the mutant S537A has no effect | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of enzyme knockout mutant HeLa cells by treatment with specific siRNA, phenotype, overview | Homo sapiens |
| additional information | generation of phosphatidic acid-preferring phospholipase A1 knock-out mice using targeting vector clone PGS00049_B_D06 and specific siRNA, phenotype, overview | Mus musculus |
| S537A | site-directed mutagenesis, inactive mutant | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Mus musculus | 5622 | - |
| intracellular | - |
Homo sapiens | 5622 | - |
| mitochondrion | - |
Mus musculus | 5739 | - |
| mitochondrion | - |
Homo sapiens | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidic acid + H2O | Mus musculus | - |
2-acyl-lysophosphatidic acid + a carboxylate | - |
? |  |
| phosphatidic acid + H2O | Homo sapiens | - |
2-acyl-lysophosphatidic acid + a carboxylate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | high expression level | Homo sapiens | - |
| caput epididymis | - |
Mus musculus | - |
| cauda epididymis | - |
Mus musculus | - |
| HeLa cell | - |
Homo sapiens | - |
| spermatozoon | - |
Mus musculus | - |
| testis | high expression level | Mus musculus | - |
| testis | high expression level | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidic acid + H2O | - |
Mus musculus | 2-acyl-lysophosphatidic acid + a carboxylate | - |
? | |
| phosphatidic acid + H2O | - |
Homo sapiens | 2-acyl-lysophosphatidic acid + a carboxylate | - |
? | |
| phosphatidic acid + H2O | preferred substrate in vitro | Mus musculus | 2-acyl-lysophosphatidic acid + a carboxylate | - |
? | |
| phosphatidic acid + H2O | preferred substrate in vitro | Homo sapiens | 2-acyl-lysophosphatidic acid + a carboxylate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PA-PLA1/DDHD1 | - |
Mus musculus |
| PA-PLA1/DDHD1 | - |
Homo sapiens |
| phosphatidic acid-preferring phospholipase A1 | - |
Mus musculus |
| phosphatidic acid-preferring phospholipase A1 | - |
Homo sapiens |
| phosphatidic acid-preferring phospholipase A1/DDHD1 | - |
Mus musculus |
| phosphatidic acid-preferring phospholipase A1/DDHD1 | - |
Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | phosphatidic acid-preferring phospholipase A1 depletion causes mitochondrial elongation, leading to a loss of motility | Homo sapiens |
| malfunction | phosphatidic acid-preferring phospholipase A1 depletion causes mitochondrial elongation. Enzyme knock-out mice have a defect in sperm formation, spermatozoa from PA-PLA1-/- mice possess an annulus that is not attached to the mitochondrial sheath, whereas that in control mice is attached. In enzyme-deficient sperm, the mitochondrial structure is disorganized, and an abnormal gap structure exists between the middle and principal pieces.A flagellum is bent at that position, leading to a loss of motility | Mus musculus |
| physiological function | the phosphatidic acid-preferring phospholipase A1 regulates mitochondrial dynamics | Mus musculus |
| physiological function | the phosphatidic acid-preferring phospholipase A1 regulates mitochondrial dynamics. When ectopically expressed in HeLa cells, it induces mitochondrial fragmentation, whereas its depletion causes mitochondrial elongation. The effects of the enzyme on mitochondrial morphology appear to counteract those of MitoPLD, a mitochondrion-localized phospholipase D that produces phosphatidic acid from cardiolipin | Homo sapiens |
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