Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Serratia sp. xJF1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cr3+ | 51.4% relative activity at 5 mM | Serratia sp. xJF1 | |
Cu2+ | 56.8% relative activity at 5 mM | Serratia sp. xJF1 | |
EDTA | complete inhibition at 5 mM | Serratia sp. xJF1 | |
Fe2+ | 89.2% relative activity at 5 mM | Serratia sp. xJF1 | |
Mg2+ | 13.5% relative activity at 5 mM | Serratia sp. xJF1 | |
Mn2+ | complete inhibition at 5 mM | Serratia sp. xJF1 | |
Na+ | 54.7% relative activity at 5 mM | Serratia sp. xJF1 | |
SDS | 20.9% relative activity at 5 mM | Serratia sp. xJF1 | |
Triton X-100 | complete inhibition at 5 mM | Serratia sp. xJF1 | |
Zn2+ | 52% relative activity at 5 mM | Serratia sp. xJF1 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 111.5% relative activity at 5 mM Ca2+ | Serratia sp. xJF1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33800 | - |
calculated from amino acid sequence | Serratia sp. xJF1 |
35000 | - |
SDS-PAGE | Serratia sp. xJF1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Serratia sp. xJF1 | A9X3M0 | - |
- |
Purification (Comment) | Organism |
---|---|
Q sepharose column chromatography | Serratia sp. xJF1 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
202.3 | - |
recombinant enzyme, after 8.5fold purification, at pH 9.0 and 35°C | Serratia sp. xJF1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + H2O | - |
Serratia sp. xJF1 | 2-acylglycerophosphocholine + a fatty acid anion | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PLA1 | - |
Serratia sp. xJF1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
recombinant enzyme | Serratia sp. xJF1 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 55 | the enzyme loses most of its activity at 55°C but retains about 70% of its maximum activity at 10°C, heat treatment at 60°C for 10 min results in an 86% decrease in enzyme activity | Serratia sp. xJF1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Serratia sp. xJF1 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10 | the enzyme is stable between pH 6 and 10 at the indicated pH range when incubated at 35°C for 3 h, but the enzyme loses about 50% of its activity at pHs below 5.5 | Serratia sp. xJF1 |