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Literature summary for 3.1.1.3 extracted from

  • Imanaka, T.; Amanuma-Muto, K.; Ohkuma, S.; Takano, T.
    Characterization of lysosomal acid lipase purified from rabbit liver (1984), J. Biochem., 96, 1089-1101.
    View publication on PubMed
Search for more literature for 3.1.1.3

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Oryctolagus cuniculus
diethyl dicarbonate
-
 Oryctolagus cuniculus
diisopropyl fluorophosphate weak Oryctolagus cuniculus
p-bromophenacyl bromide
-
 Oryctolagus cuniculus
p-chloromercuriphenyl sulfonic acid
-
 Oryctolagus cuniculus
phenylmethylsulfonyl fluoride weak Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 4-methylumbelliferyl oleate
-
 Oryctolagus cuniculus
0.04
-
 cholesterol oleate
-
 Oryctolagus cuniculus
0.05
-
 triolein
-
 Oryctolagus cuniculus
0.07
-
 diolein
-
 Oryctolagus cuniculus
0.2
-
 monoolein
-
 Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Oryctolagus cuniculus 5764
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Oryctolagus cuniculus
42000
-
 1 * 42000, SDS-PAGE Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Oryctolagus cuniculus the enzyme is involved in the hydrolysis of both acylglycerols and cholesterol esters in lysosomes ?
-
 ?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification hydrophobic glycoprotein with variations in its carbohydrate chains Oryctolagus cuniculus

Purification (Commentary)

Purification (Comment) Organism
-
 Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
216
-
-
 Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
-70°C, in presence of 40% v/v ethylene glycol and 5 mM mercaptoethanol, stable for at least 1 month Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl ester + H2O with fatty acids of different length in decreasing order, medium chain, long chains, short chains Oryctolagus cuniculus 4-methylumbelliferone + fatty acid
-
 ?
4-methylumbelliferyl oleate + H2O
-
 Oryctolagus cuniculus 4-methylumbelliferone + oleic acid
-
 ?
cholesterol oleate + H2O
-
 Oryctolagus cuniculus cholesterol + oleic acid
-
 ?
diolein + H2O
-
 Oryctolagus cuniculus oleic acid + ?
-
 ?
monoolein + H2O
-
 Oryctolagus cuniculus oleic acid + glycerol
-
 ?
additional information the enzyme is involved in the hydrolysis of both acylglycerols and cholesterol esters in lysosomes Oryctolagus cuniculus ?
-
 ?
trioleoylglycerol + H2O
-
 Oryctolagus cuniculus oleic acid + ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 42000, SDS-PAGE Oryctolagus cuniculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 10 min, 50% inactivation Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3
-
 hydrolysis of cholesterol oleate Oryctolagus cuniculus
4.5
-
 hydrolysis of triolein, diolein or monoolein Oryctolagus cuniculus
5
-
 hydrolysis of 4-methylumbelliferyl oleate Oryctolagus cuniculus