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Literature summary for 3.1.1.3 extracted from
- Purification and general properties of a metal-insensitive lipase from Rhizopus japonicus NR 400 (1986), J. Biochem., 100, 1207-1213.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| albumin | 0.01%, enhances activity between pH 3 and 8 | Rhizopus japonicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
gel filtration | Rhizopus japonicus |
| 30000 | - |
1 * 30000, SDS-PAGE | Rhizopus japonicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizopus japonicus | - |
NR 400 | - |
| Rhizopus japonicus NR 400 | - |
NR 400 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhizopus japonicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rhizopus japonicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stable for at least several months | Rhizopus japonicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tricaprin + H2O | - |
Rhizopus japonicus | ? | - |
? |  |
| tricaprin + H2O | - |
Rhizopus japonicus NR 400 | ? | - |
? | |
| tricaprylin + H2O | - |
Rhizopus japonicus | ? | - |
? | |
| tricaprylin + H2O | - |
Rhizopus japonicus NR 400 | ? | - |
? | |
| tripalmitin + H2O | - |
Rhizopus japonicus | palmitic acid + ? | - |
? | |
| tripalmitin + H2O | - |
Rhizopus japonicus NR 400 | palmitic acid + ? | - |
? | |
| tristearin + H2O | - |
Rhizopus japonicus | stearic acid + ? | - |
? | |
| tristearin + H2O | - |
Rhizopus japonicus NR 400 | stearic acid + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 30000, SDS-PAGE | Rhizopus japonicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
30 min, 50% loss of activity | Rhizopus japonicus |
| 64 | - |
30 min, in presence of substrates, 50% loss of activity | Rhizopus japonicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Rhizopus japonicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | 50% of maximal activity at pH 3 and 7 | Rhizopus japonicus |
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