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Literature summary for 3.1.1.3 extracted from

  • Kumari, A.; Gupta, R.
    Functional characterization of a novel aspartic acid rich lipase, TALipC, from Trichosporon asahii MSR54: solvent-dependent enantio inversion during esterification of 1-phenylethanol (2015), Biotechnol. Lett., 37, 121-130.
    View publication on PubMed
Search for more literature for 3.1.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris X-33 cells Trichosporon asahii

Inhibitors

Inhibitors Comment Organism Structure
EDTA almost complete inhibition at 10 mM Trichosporon asahii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activated by Mg2+ ions (20 mM), about 140% activity at 25 mM Mg2+ Trichosporon asahii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 x * 54000, SDS-PAGE Trichosporon asahii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
triacylglycerol + H2O Trichosporon asahii
-
 diacylglycerol + a carboxylate
-
 ?
triacylglycerol + H2O Trichosporon asahii MSR54
-
 diacylglycerol + a carboxylate
-
 ?

Organism

Organism UniProt Comment Textmining
Trichosporon asahii
-
-
-
Trichosporon asahii MSR54
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA affinity column chromatography, and gel filtration Trichosporon asahii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl palmitate + H2O
-
 Trichosporon asahii 4-nitrophenol + palmitate
-
 ?
4-nitrophenyl palmitate + H2O
-
 Trichosporon asahii MSR54 4-nitrophenol + palmitate
-
 ?
additional information the enzyme reveals mid- to long- chain specificity for 4-nitrophenyl esters and triacylglycerols, with highest specificity on C-12 followed by C-14, C-16, and C-18. The enzyme shows (S)-selectivity in 1,4-dioxane and 2-propanol and (R)-selectivity in hexane during chiral separation of (+/-)1-phenylethanol by esterification Trichosporon asahii ?
-
 ?
additional information the enzyme reveals mid- to long- chain specificity for 4-nitrophenyl esters and triacylglycerols, with highest specificity on C-12 followed by C-14, C-16, and C-18. The enzyme shows (S)-selectivity in 1,4-dioxane and 2-propanol and (R)-selectivity in hexane during chiral separation of (+/-)1-phenylethanol by esterification Trichosporon asahii MSR54 ?
-
 ?
triacylglycerol + H2O
-
 Trichosporon asahii diacylglycerol + a carboxylate
-
 ?
triacylglycerol + H2O
-
 Trichosporon asahii MSR54 diacylglycerol + a carboxylate
-
 ?

Subunits

Subunits Comment Organism
? x * 54000, SDS-PAGE Trichosporon asahii

Synonyms

Synonyms Comment Organism
TALipC
-
 Trichosporon asahii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Trichosporon asahii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 70 about 80% activity at 10°C, about 85% activity at 20°C, about 90% activity at 30°C, about 97% activity at 40°C, 100% activity at 50°C, about 90% activity at 60°C, about 55% activity at 70°C Trichosporon asahii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Trichosporon asahii

pH Range

pH Minimum pH Maximum Comment Organism
6 11 about 58% activity at pH 6.0, about 90% activity at pH 7.0, 100% activity at pH 9.0, about 78% activity at pH 10.0, about 60% activity at pH 11.0 Trichosporon asahii