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Literature summary for 3.1.1.3 extracted from

  • Dahiya, P.; Arora, P.; Chaudhury, A.; Chand, S.; Dilbaghi, N.
    Characterization of an extracellular alkaline lipase from Pseudomonas mendocina M-37 (2010), J. Basic Microbiol., 50, 420-426.
    View publication on PubMed
Search for more literature for 3.1.1.3

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 62.5% residual activity at 1 mM Pseudomonas mendocina
Cu2+ 37.5% residual activity at 1 mM Pseudomonas mendocina
EDTA 92% residual activity at 1 mM Pseudomonas mendocina
Fe2+ 71.37% residual activity at 1 mM Pseudomonas mendocina
Hg2+ 43.75% residual activity at 1 mM Pseudomonas mendocina
K+ 75% residual activity at 1 mM Pseudomonas mendocina
Mn2+ 75% residual activity at 1 mM Pseudomonas mendocina
SDS 44% residual activity at 0.1% (w/v) after 15 min incubation, 24.3% residual activity at 0.1% (w/v) after 30 min incubation, 5.3% residual activity at 0.1% (w/v) after 45 min incubation Pseudomonas mendocina
Triton X-100 91% residual activity at 1 mM Pseudomonas mendocina
Tween 20 40% residual activity at 1 mM Pseudomonas mendocina
Tween 80 60% residual activity at 1 mM Pseudomonas mendocina
Zn2+ 50% residual activity at 1 mM Pseudomonas mendocina

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pseudomonas mendocina
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 183.3% activity at 1 mM Pseudomonas mendocina
Mg2+ 156.25% activity at 1 mM Pseudomonas mendocina
Na+ 102% activity at 1 mM Pseudomonas mendocina

Organic Solvent Stability

Organic Solvent Comment Organism
1-octanol 215% activity at 20% (v/v) Pseudomonas mendocina
Acetone 150% activity at 20% (v/v) Pseudomonas mendocina
chloroform 400% activity at 20% (v/v) Pseudomonas mendocina
Ethanol 100% activity at 20% (v/v) Pseudomonas mendocina
Methanol 150% activity at 20% (v/v) Pseudomonas mendocina
n-hexane 150% activity at 60% (v/v) Pseudomonas mendocina
propanol 100% activity at 20% (v/v) Pseudomonas mendocina
toluene 250% activity at 20% (v/v) Pseudomonas mendocina

Organism

Organism UniProt Comment Textmining
Pseudomonas mendocina
-
-
-
Pseudomonas mendocina M-37
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amyl acetate + H2O 7% activity compared to olive oil Pseudomonas mendocina ?
-
 ?
amyl butyrate + H2O 550% activity compared to olive oil Pseudomonas mendocina ?
-
 ?
amyl propionate + H2O 40% activity compared to olive oil Pseudomonas mendocina ?
-
 ?
benzyl butyrate + H2O highest activity, 1120% activity compared to olive oil Pseudomonas mendocina ?
-
 ?
benzyl propionate + H2O 950% activity compared to olive oil Pseudomonas mendocina benzyl alcohol + propionate
-
 ?
benzyl propionate + H2O 950% activity compared to olive oil Pseudomonas mendocina M-37 benzyl alcohol + propionate
-
 ?
benzyl salicylate + H2O 100% activity compared to olive oil Pseudomonas mendocina ?
-
 ?
ethyl butyrate + H2O 245% activity compared to olive oil Pseudomonas mendocina ?
-
 ?
additional information the enzyme also uses olive oil (100% activity), mustard oil (96% activity), soybean oil (88.8% activity), palm oil (73% activity), and groundnut oil (92% activity) as substrates Pseudomonas mendocina ?
-
 ?
additional information the lipase possesses 1,3-positional specificity Pseudomonas mendocina ?
-
 ?
additional information the enzyme also uses olive oil (100% activity), mustard oil (96% activity), soybean oil (88.8% activity), palm oil (73% activity), and groundnut oil (92% activity) as substrates Pseudomonas mendocina M-37 ?
-
 ?
additional information the lipase possesses 1,3-positional specificity Pseudomonas mendocina M-37 ?
-
 ?
triacetin + H2O 368% activity compared to olive oil Pseudomonas mendocina diacetin + acetate
-
 ?
tributyrin + H2O 744% activity compared to olive oil Pseudomonas mendocina dibutyrin + butyrate
-
 ?
tributyrin + H2O 744% activity compared to olive oil Pseudomonas mendocina M-37 dibutyrin + butyrate
-
 ?
tricaprylin + H2O 89% activity compared to olive oil Pseudomonas mendocina dicaprylin + caprylate
-
 ?
triolein + H2O 110% activity compared to olive oil Pseudomonas mendocina diolein + oleate
-
 ?
triolein + H2O 110% activity compared to olive oil Pseudomonas mendocina M-37 diolein + oleate
-
 ?

Synonyms

Synonyms Comment Organism
lipase
-
 Pseudomonas mendocina
triacylglycerolhydrolase
-
 Pseudomonas mendocina

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Pseudomonas mendocina

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 65
-
 Pseudomonas mendocina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Pseudomonas mendocina

pH Range

pH Minimum pH Maximum Comment Organism
7 11
-
 Pseudomonas mendocina

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1
-
 pH 9.0, 50°C Pseudomonas mendocina Zn2+