Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbic acid | 124.4% activity at 1 mM | Amycolatopsis mediterranei | |
beta-mercaptoethanol | 136.4% activity at 1 mM | Amycolatopsis mediterranei | |
Digitonin | 142.5% activity at 1 mM | Amycolatopsis mediterranei | |
dithiothreitol | 147.6% activity at 1 mM | Amycolatopsis mediterranei | |
sodium deoxycholate | 241.3% activity at 1 mM | Amycolatopsis mediterranei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide | complete inhibition at 5 mM | Amycolatopsis mediterranei | |
capric acid | 89.7% residual activity at 1 mM | Amycolatopsis mediterranei | |
diethyldicarbonate | complete inhibition at 5 mM | Amycolatopsis mediterranei | |
Hg2+ | Hg2+ shows the highest reduction in enzyme activity by 83.3% at 1 mM | Amycolatopsis mediterranei | |
additional information | not affected by EDTA, sodium citrate, SDS, myristic acid, palmitic acid, and urea (6.0 M). The enzyme is not inhibited by iodoacetate, citraconic anhydride, phenylglyoxal, and N-acetylimidazole | Amycolatopsis mediterranei | |
N-bromosuccinimide | 34.4% residual activity at 5 mM | Amycolatopsis mediterranei | |
phenylmethylsulfonyl fluoride | 11.6% residual activity at 5 mM | Amycolatopsis mediterranei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.099 | - |
4-nitrophenyl palmitate | pH 8.0, 60°C | Amycolatopsis mediterranei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 33000, SDS-PAGE | Amycolatopsis mediterranei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amycolatopsis mediterranei | - |
- |
- |
Amycolatopsis mediterranei DSM 43304 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Q Sepharose column chromatography, and Toyopearl phenyl-650M column chromatography (398fold purification with 36% final recovery) | Amycolatopsis mediterranei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.96 | - |
enzyme from culture filtrate, using 4-nitrophenyl palmitate as substrate, at pH 8.0 and 60°C | Amycolatopsis mediterranei |
781.6 | - |
enzyme after 398.79fold purification, using 4-nitrophenyl palmitate as substrate, at pH 8.0 and 60°C | Amycolatopsis mediterranei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 triolein + H2O | about 115% activity compared to olive oil | Amycolatopsis mediterranei | 6 oleic acid + 1,3-diolein + 1,2-diolein + 1-monoolein + 2-monoolein | oleic acid is the major product | ? | |
4-nitrophenyl acetate + H2O | about 88% activity compared to 4-nitrophenyl palmitate | Amycolatopsis mediterranei | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | about 88% activity compared to 4-nitrophenyl palmitate | Amycolatopsis mediterranei DSM 43304 | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | about 90% activity compared to 4-nitrophenyl palmitate | Amycolatopsis mediterranei | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | about 90% activity compared to 4-nitrophenyl palmitate | Amycolatopsis mediterranei DSM 43304 | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl caproate + H2O | second-highest hydrolysis rate, about 95% activity compared to 4-nitrophenyl palmitate | Amycolatopsis mediterranei | 4-nitrophenol + caproate | - |
? | |
4-nitrophenyl caproate + H2O | second-highest hydrolysis rate, about 95% activity compared to 4-nitrophenyl palmitate | Amycolatopsis mediterranei DSM 43304 | 4-nitrophenol + caproate | - |
? | |
4-nitrophenyl caprylate + H2O | highest hydrolysis rate, about 90% activity compared to 4-nitrophenyl palmitate | Amycolatopsis mediterranei | 4-nitrophenol + caprylate | - |
? | |
4-nitrophenyl laurate + H2O | about 60% activity compared to 4-nitrophenyl palmitate | Amycolatopsis mediterranei | 4-nitrophenol + laurate | - |
? | |
4-nitrophenyl myristate + H2O | about 30% activity compared to 4-nitrophenyl palmitate | Amycolatopsis mediterranei | 4-nitrophenol + myristate | - |
? | |
4-nitrophenyl palmitate + H2O | the purified enzyme is most active toward 4-nitrophenyl palmitate (100% activity) | Amycolatopsis mediterranei | 4-nitrophenol + palmitate | - |
? | |
isoamyl alcohol + acetic acid | after 72 h of reaction a conversion yield of 34.4% and 16.2% of isoamyl acetate is obtained using cellite-immobilized and free enzyme, respectively | Amycolatopsis mediterranei | isoamyl acetate | - |
? | |
additional information | the enzyme also uses olive oil (100% activity), corn oil (about 85% activity), castor oil (about 80% activity), sunflower oil (about 95% activity), rape seed oil (about 85% activity), linseed oil (about 75% activity), cotton seed (about 90% activity), and jojoba oil (about 83% activity) as substrates | Amycolatopsis mediterranei | ? | - |
? | |
additional information | the enzyme also uses olive oil (100% activity), corn oil (about 85% activity), castor oil (about 80% activity), sunflower oil (about 95% activity), rape seed oil (about 85% activity), linseed oil (about 75% activity), cotton seed (about 90% activity), and jojoba oil (about 83% activity) as substrates | Amycolatopsis mediterranei DSM 43304 | ? | - |
? | |
trilinolein + H2O | about 105% activity compared to olive oil | Amycolatopsis mediterranei | ? | - |
? | |
trilinolenin + H2O | about 100% activity compared to olive oil | Amycolatopsis mediterranei | ? | - |
? | |
trioctanoin + H2O | about 59% activity compared to olive oil | Amycolatopsis mediterranei | ? | - |
? | |
trioctanoin + H2O | about 59% activity compared to olive oil | Amycolatopsis mediterranei DSM 43304 | ? | - |
? | |
tripalmitin + H2O | about 45% activity compared to olive oil | Amycolatopsis mediterranei | ? | - |
? | |
tristearin + H2O | about 38% activity compared to olive oil | Amycolatopsis mediterranei | ? | - |
? | |
trivaccinin + H2O | about 78% activity compared to olive oil | Amycolatopsis mediterranei | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000, SDS-PAGE | Amycolatopsis mediterranei |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Amycolatopsis mediterranei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Amycolatopsis mediterranei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | the purified enzyme is most stable at 50-60°C retaining 100% activity after 3 h | Amycolatopsis mediterranei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1468 | - |
4-nitrophenyl palmitate | apparent value, in 50 mM Tris-HCl buffer, at pH 8.0, 60°C | Amycolatopsis mediterranei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Amycolatopsis mediterranei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | the activity is not much affected at pH 7.0 and 9.0 where it shows around 90% of relative activity | Amycolatopsis mediterranei |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | the purified enzyme is stable in the pH range 6.0-9.0 retaining more than 95% of relative activity after 24 h of incubation | Amycolatopsis mediterranei |