Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | complete inhibition at 10 mM | Bacillus sp. (in: Bacteria) | |
Ni2+ | 78% inhibition at 10 mM | Bacillus sp. (in: Bacteria) | |
phenylmethylsulfonyl fluoride | complete inhibition at 10 mM | Bacillus sp. (in: Bacteria) | |
Zn2+ | 86% inhibition at 10 mM | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | the activity is enhanced more than twice at 1 mM CoCl2 | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22800 | - |
x * 22800, calculated from amino acid sequence | Bacillus sp. (in: Bacteria) |
23000 | - |
x * 23000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Bacillus sp. (in: Bacteria) HH-01 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl octanoate + H2O | - |
Bacillus sp. (in: Bacteria) | 1-naphthol + octanoate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Bacillus sp. (in: Bacteria) | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Bacillus sp. (in: Bacteria) HH-01 | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl caprylate + H2O | at 30°C, the enzyme prefers C8 synthetic substrate (4-nitrophenyl caprylate)to shorter or longer substrates | Bacillus sp. (in: Bacteria) | 4-nitrophenol + caprylate | - |
? | |
4-nitrophenyl caprylate + H2O | at 30°C, the enzyme prefers C8 synthetic substrate (4-nitrophenyl caprylate)to shorter or longer substrates | Bacillus sp. (in: Bacteria) HH-01 | 4-nitrophenol + caprylate | - |
? | |
4-nitrophenyl laurate + H2O | - |
Bacillus sp. (in: Bacteria) | 4-nitrophenol + laurate | - |
? | |
4-nitrophenyl laurate + H2O | - |
Bacillus sp. (in: Bacteria) HH-01 | 4-nitrophenol + laurate | - |
? | |
4-nitrophenyl palmitate + H2O | - |
Bacillus sp. (in: Bacteria) | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl palmitate + H2O | - |
Bacillus sp. (in: Bacteria) HH-01 | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl stearate + H2O | - |
Bacillus sp. (in: Bacteria) | 4-nitrophenol + stearate | - |
? | |
triacylglycerol + H2O | the strain HH-01 degrades 1.0% (v/v) edible oil in nutrient broth, almost completely within 10 days | Bacillus sp. (in: Bacteria) | diacylglycerol + a carboxylate | - |
? | |
triacylglycerol + H2O | the strain HH-01 degrades 1.0% (v/v) edible oil in nutrient broth, almost completely within 10 days | Bacillus sp. (in: Bacteria) HH-01 | diacylglycerol + a carboxylate | - |
? | |
trilinolein + H2O | the enzyme shows preferential hydrolysis of the 1(3)-position ester bond in trilinolein | Bacillus sp. (in: Bacteria) | dilinolein + linoleate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
? | x * 22800, calculated from amino acid sequence | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
LipA | - |
Bacillus sp. (in: Bacteria) |
lipase | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
the enzyme is stable up to 30°C | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | - |
Bacillus sp. (in: Bacteria) |
Organism | Comment | Expression |
---|---|---|
Bacillus sp. (in: Bacteria) | the lipA gene is down-regulated in the stationary phase | down |
Bacillus sp. (in: Bacteria) | the lipA gene is constitutively transcribed during the exponential growth phase | up |