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Literature summary for 3.1.1.3 extracted from

  • Kamijo, T.; Saito, A.; Ema, S.; Yoh, I.; Hayashi, H.; Nagata, R.; Nagata, Y.; Ando, A.
    Molecular and enzymatic characterization of a subfamily I.4 lipase from an edible oil-degrader Bacillus sp. HH-01 (2011), Antonie van Leeuwenhoek, 99, 179-187.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ complete inhibition at 10 mM Bacillus sp. (in: Bacteria)
Ni2+ 78% inhibition at 10 mM Bacillus sp. (in: Bacteria)
phenylmethylsulfonyl fluoride complete inhibition at 10 mM Bacillus sp. (in: Bacteria)
Zn2+ 86% inhibition at 10 mM Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ the activity is enhanced more than twice at 1 mM CoCl2 Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22800
-
x * 22800, calculated from amino acid sequence Bacillus sp. (in: Bacteria)
23000
-
x * 23000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) HH-01
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl octanoate + H2O
-
Bacillus sp. (in: Bacteria) 1-naphthol + octanoate
-
?
4-nitrophenyl butyrate + H2O
-
Bacillus sp. (in: Bacteria) 4-nitrophenol + butyrate
-
?
4-nitrophenyl butyrate + H2O
-
Bacillus sp. (in: Bacteria) HH-01 4-nitrophenol + butyrate
-
?
4-nitrophenyl caprylate + H2O at 30°C, the enzyme prefers C8 synthetic substrate (4-nitrophenyl caprylate)to shorter or longer substrates Bacillus sp. (in: Bacteria) 4-nitrophenol + caprylate
-
?
4-nitrophenyl caprylate + H2O at 30°C, the enzyme prefers C8 synthetic substrate (4-nitrophenyl caprylate)to shorter or longer substrates Bacillus sp. (in: Bacteria) HH-01 4-nitrophenol + caprylate
-
?
4-nitrophenyl laurate + H2O
-
Bacillus sp. (in: Bacteria) 4-nitrophenol + laurate
-
?
4-nitrophenyl laurate + H2O
-
Bacillus sp. (in: Bacteria) HH-01 4-nitrophenol + laurate
-
?
4-nitrophenyl palmitate + H2O
-
Bacillus sp. (in: Bacteria) 4-nitrophenol + palmitate
-
?
4-nitrophenyl palmitate + H2O
-
Bacillus sp. (in: Bacteria) HH-01 4-nitrophenol + palmitate
-
?
4-nitrophenyl stearate + H2O
-
Bacillus sp. (in: Bacteria) 4-nitrophenol + stearate
-
?
triacylglycerol + H2O the strain HH-01 degrades 1.0% (v/v) edible oil in nutrient broth, almost completely within 10 days Bacillus sp. (in: Bacteria) diacylglycerol + a carboxylate
-
?
triacylglycerol + H2O the strain HH-01 degrades 1.0% (v/v) edible oil in nutrient broth, almost completely within 10 days Bacillus sp. (in: Bacteria) HH-01 diacylglycerol + a carboxylate
-
?
trilinolein + H2O the enzyme shows preferential hydrolysis of the 1(3)-position ester bond in trilinolein Bacillus sp. (in: Bacteria) dilinolein + linoleate
-
?

Subunits

Subunits Comment Organism
? x * 23000, SDS-PAGE Bacillus sp. (in: Bacteria)
? x * 22800, calculated from amino acid sequence Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
LipA
-
Bacillus sp. (in: Bacteria)
lipase
-
Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
the enzyme is stable up to 30°C Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 11
-
Bacillus sp. (in: Bacteria)

Expression

Organism Comment Expression
Bacillus sp. (in: Bacteria) the lipA gene is down-regulated in the stationary phase down
Bacillus sp. (in: Bacteria) the lipA gene is constitutively transcribed during the exponential growth phase up