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Literature summary for 3.1.1.3 extracted from
- Purification and characterization of the first reptile pancreatic lipase: the turtle (2008), Pancreas, 36, 302-308.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not affected by EDTA and Ca2+ | Testudo graeca nabeulensis | |
| sodium deoxycholate | completely inhibited at 4 mM sodium deoxycholate | Testudo graeca nabeulensis |  |
| sodium taurodeoxycholate | the inhibition is reversed by the addition of colipase | Testudo graeca nabeulensis | |
| tetrahydrolipstatine | 40% residual activity in the absence of sodium deoxycholate, complete inhibition in the presence of 4 mM sodium deoxycholate | Testudo graeca nabeulensis | |
| Triton X-100 | the activity of the enzyme dereases rapidly with a concentration of Triton X-100 greater than 0.3 mM | Testudo graeca nabeulensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
SDS-PAGE | Testudo graeca nabeulensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Testudo graeca nabeulensis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-cellulose column chromatography, Sephacryl S-200 gel filtration, and Mono-Q Sepharose column chromatography | Testudo graeca nabeulensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Testudo graeca nabeulensis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| olive oil + H2O | - |
Testudo graeca nabeulensis | ? | - |
? | |
| tributyrin + H2O | - |
Testudo graeca nabeulensis | dibutyrin + butyrate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glycerol ester hydrolase | - |
Testudo graeca nabeulensis |
| Pancreatic lipase | - |
Testudo graeca nabeulensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Testudo graeca nabeulensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
the enzyme is inactivated at temperatures higher than 50°C after few min of incubation | Testudo graeca nabeulensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
- |
Testudo graeca nabeulensis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | stable at pH 7-9, loses its activity at pH lower 4 after 1 h of incubation | Testudo graeca nabeulensis |
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Release 2023.1 (January 2023)
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