General Stability | Organism |
---|---|
immobilization by adsorption on porous polypropylene (Accurel EP-100) increases enzyme thermostability (shows residual activity even after 1 h at 100°C, when soluble enzyme completely loses activity after 1 h at 80°C) but there is no change in neither the optimum pH nor in pH resistance irrelevant to the presence of Triton X-100 during immobilization | Geobacillus thermoleovorans |
Organic Solvent | Comment | Organism |
---|---|---|
SDS | activity is completely lost with SDS | Geobacillus thermoleovorans |
Triton X-100 | free enzyme shows less than 50% loss of activity with 0.1% (v/v) Triton X-100, lipase immobilized in the presence of Triton X-100 shows a total loss of activity after 9 cycles of reuse, retaining 53% of its initial activity after seven cycles, while the immobilized lipase without Triton X-100 loses all of its activity after 12 cycles, retaining 69% of its initial activity after 7 cycles | Geobacillus thermoleovorans |
Tween 80 | less than 50% loss of activity with 0.1% (v/v) Tween 80 | Geobacillus thermoleovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermoleovorans | Q0JRM8 | - |
- |
Geobacillus thermoleovorans CCR11 | Q0JRM8 | - |
- |
Purification (Comment) | Organism |
---|---|
partially purified by diafiltration | Geobacillus thermoleovorans |
Storage Stability | Organism |
---|---|
4°C, immobilized enzyme (Accurel EP-100), 36 days, immobilized lipase shows less activity over short chain esters than the soluble lipase | Geobacillus thermoleovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl laurate + H2O | - |
Geobacillus thermoleovorans | 4-nitrophenol + laurate | - |
? | |
4-nitrophenyl laurate + H2O | - |
Geobacillus thermoleovorans CCR11 | 4-nitrophenol + laurate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Geobacillus thermoleovorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 100 | free enzyme retains 100% activity after 1 h at 50°C, immobilized enzyme retains 100% activity after 1 h at 60°C, free enzyme shows 13% residual activity after 1 h at 80°C, immobilized enzyme retains 15.2% residual activity after 1 h at 100°C | Geobacillus thermoleovorans |