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Literature summary for 3.1.1.3 extracted from
- An extra-cellular alkaline metallolipase from Bacillus licheniformis MTCC 6824: Purification and biochemical characterization (2008), Food Chem., 109, 727-736.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sorbitol | a combination of Ca2+ and sorbitol induces a synergistic effect on the activity of lipase with a significantly high residual activity (100%), even after 45 min, as compared to 91.5% when incubated with Ca2+ alone | Bacillus licheniformis |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Bacillus licheniformis | |
| acetonitrile | inhibitory at 5% (v/v) | Bacillus licheniformis | |
| chloroform | inhibitory at 3% (v/v) | Bacillus licheniformis | |
| Co2+ | 82.7% inhibition at 50 mM | Bacillus licheniformis | |
| Cu2+ | 31% inhibition at 10 mM | Bacillus licheniformis | |
| EDTA | significant inhibition at 70 mM | Bacillus licheniformis | |
| ethyl acetate | inhibitory at 8% (v/v) | Bacillus licheniformis | |
| Fe2+ | 33% inhibition at 10 mM | Bacillus licheniformis | |
| Hg2+ | 72% inhibition at 70 mM | Bacillus licheniformis | |
| additional information | Na+ has no significant effect on the enzyme activity; the activity is strongly affected by the thiol inhibitor N-bromosuccinimide | Bacillus licheniformis | |
| phenylmethylsulfonyl fluoride | completely inactivates the original lipase at 70 mM | Bacillus licheniformis | |
| Zn2+ | 69.1% inhibition at 50 mM | Bacillus licheniformis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.3 | - |
triolein | at 45°C | Bacillus licheniformis | |
| 12 | - |
tristearin | at 45°C | Bacillus licheniformis | |
| 15 | - |
tripalmitin | at 45°C | Bacillus licheniformis | |
| 22 | - |
trilaurin | at 45°C | Bacillus licheniformis | |
| 29 | - |
4-nitrophenyl palmitate | at 45°C | Bacillus licheniformis | |
| 34 | - |
tributyrin | at 45°C | Bacillus licheniformis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | the catalytic activity is enhanced by Ca2+ (18%) at 30 mM and higher concentrations | Bacillus licheniformis | |
| Mg2+ | the catalytic activity is enhanced by Mg2+ (12%) at 30 mM and higher concnetrations | Bacillus licheniformis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 74800 | - |
apparent molecular mass, non-denaturing PAGE | Bacillus licheniformis |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| isopropanol | relative activity of 62% is apparent in the presence of 1% (v/v) isopropanol | Bacillus licheniformis |
| Methanol | relative activity of 71% is apparent in the presence of 1% (v/v) methanol | Bacillus licheniformis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | - |
- |
- |
| Bacillus licheniformis MTCC 6824 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate fractionation, ethanol/ether precipitation, dialysis, Amberlite IRA 410 chromatography, and Sephadex G 100 gel filtration | Bacillus licheniformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl palmitate + H2O | - |
Bacillus licheniformis | 4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl palmitate + H2O | - |
Bacillus licheniformis MTCC 6824 | 4-nitrophenol + palmitate | - |
? | |
| glyceryl trioleate + 3 H2O | 14% activity compared to glyceryl tributyrate | Bacillus licheniformis | glycerol + 3 oleate | - |
? | |
| glyceryl trioleate + 3 H2O | 14% activity compared to glyceryl tributyrate | Bacillus licheniformis MTCC 6824 | glycerol + 3 oleate | - |
? | |
| tributyrin + 3 H2O | 100% activity | Bacillus licheniformis | glycerol + 3 butyrate | - |
? | |
| tributyrin + 3 H2O | 100% activity | Bacillus licheniformis MTCC 6824 | glycerol + 3 butyrate | - |
? | |
| trilaurin + 3 H2O | 66% activity compared to glyceryl tributyrate | Bacillus licheniformis | glycerol + 3 laurate | - |
? | |
| trilaurin + 3 H2O | 66% activity compared to glyceryl tributyrate | Bacillus licheniformis MTCC 6824 | glycerol + 3 laurate | - |
? | |
| triolein + H2O | - |
Bacillus licheniformis | 1,2-diolein + oleate | - |
? | |
| triolein + H2O | - |
Bacillus licheniformis MTCC 6824 | 1,2-diolein + oleate | - |
? | |
| tripalmitin + 3 H2O | 42% activity compared to glyceryl tributyrate | Bacillus licheniformis | glycerol + 3 palmitate | - |
? | |
| tristearin + 3 H2O | 31% activity compared to glyceryl tributyrate | Bacillus licheniformis | glycerol + 3 stearate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 74800, non-denaturing PAGE | Bacillus licheniformis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| triacylglycerol acyl hydrolase | - |
Bacillus licheniformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Bacillus licheniformis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 65 | half-lives of 82 min at 45°C and 48 min at 55°C, the activity is reduced to 63.9% of its original value in 20 min at 50°C, the enzyme retains 81.9% of the residual activity after 60 min of incubation at 45°C, and 69.3% after 2 h, at higher temperatures, e.g. 50 and 55°C, the lipase exhibits 69% and 59% of the maximum activity after 1 h of incubation, and 43% and 32% of the residual activity after 2 h of incubation, lipase exhibits significant activity even at 65°C (48% of the residual activity) after 1 h of incubation | Bacillus licheniformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bacillus licheniformis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | - |
Bacillus licheniformis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 11 | 67.9% activity at pH 9.0, 22.6% activity at pH 10.0, the activity is reduced drastically at pH 5.0 (15.5%) and is 1.94% of the maximum at pH 11.0, the enzyme is inactivated at acidic pH (4.0) losing 97.0% of its original activity, at pH 3.0, the enzyme loses about 99.7% of its initial activity | Bacillus licheniformis |
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