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Literature summary for 3.1.1.3 extracted from
- Purification and properties of the alkaline lipase from Burkholderia cepacia A.T.C.C. 25609 (2008), Biotechnol. Appl. Biochem., 51, 23-31.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| olive oil | best inducer for the production of lipase | Burkholderia cepacia |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain ER2566 | Burkholderia cepacia |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11 | - |
4-nitrophenyl palmitate | at pH 7 and 37°C | Burkholderia cepacia |  |
| 16.6 | - |
tributyrin | at pH 7 and 37°C | Burkholderia cepacia | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
SDS-PAGE | Burkholderia cepacia |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Triton X-100 | stable in the presence of the detergent Triton X-100 | Burkholderia cepacia |
| Tween | stable in the presence of the detergent Tween 20 | Burkholderia cepacia |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia cepacia | A9QXC9 | strain ATCC 25609, formerly called Pseudomonas cepacia | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation and Macro-Prep methyl column chromatography | Burkholderia cepacia |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 230 | - |
crude extract, at pH 7 and 37°C | Burkholderia cepacia |
| 8830 | - |
after 38fold purification, at pH 7 and 37°C | Burkholderia cepacia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl palmitate + H2O | - |
Burkholderia cepacia | 4-nitrophenol + palmitate | - |
? | |
| tributyrin + H2O | - |
Burkholderia cepacia | dibutyrin + butyrate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipase | - |
Burkholderia cepacia |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Burkholderia cepacia |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | half-life is 54 and 46 min at 50 and 60°C respectively | Burkholderia cepacia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Burkholderia cepacia |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10 | stable up to 12 h at pH 9 and 10, loses up to 50% activity after 12 h at pH 8 | Burkholderia cepacia |
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