Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Geobacillus thermocatenulatus |
General Stability | Organism |
---|---|
the aminated enzyme immobilized on glyoxyl-agarose by multipoint covalent attachment retains 65% of the starting activity, the optimal stabilization protocol is the immobilization of aminated BTL2 at pH 9 and the further incubation for 24 h at 25°C and pH 10, this preparation is 5fold more stable than the optimal BTL2 immobilized on glyoxyl agarose and around 1200fold more stable than the enzyme immobilized on CNBr and further aminated | Geobacillus thermocatenulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermocatenulatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
butyl-Sepharose column chromatography | Geobacillus thermocatenulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,S)-2-O-butyryl-2-phenylacetate + H2O | Enantioselectivity of the reaction can be obtained with various methods of enzyme immoblisation and reaction conditions | Geobacillus thermocatenulatus | (R)-2-O-butyryl-2-phenylacetate + (2S)-hydroxy(phenyl)ethanoic acid + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Geobacillus thermocatenulatus | 4-nitrophenol + butyrate | - |
? | |
diethyl 3-phenylglutarate + H2O | - |
Geobacillus thermocatenulatus | (S)-ethyl phenylglutarate + (R)-ethyl phenylglutarate + ethanol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BTL2 | - |
Geobacillus thermocatenulatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
the optimal preparation retains 100% of its activity after 100 h of incubation at pH 7 and 65°C | Geobacillus thermocatenulatus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
the optimal preparation retains 100% of its activity after 100 h of incubation at pH 7 and 65°C | Geobacillus thermocatenulatus |