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Literature summary for 3.1.1.3 extracted from

  • Fernandez-Lorente, G.; Godoy, C.A.; Mendes, A.A.; Lopez-Gallego, F.; Grazu, V.; de Las Rivas, B.; Palomo, J.M.; Hermoso, J.; Fernandez-Lafuente, R.; Guisan, J.M.
    Solid-phase chemical amination of a lipase from Bacillus thermocatenulatus to improve its stabilization via covalent immobilization on highly activated glyoxyl-agarose (2008), Biomacromolecules, 9, 2553-2561.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Geobacillus thermocatenulatus

General Stability

General Stability Organism
the aminated enzyme immobilized on glyoxyl-agarose by multipoint covalent attachment retains 65% of the starting activity, the optimal stabilization protocol is the immobilization of aminated BTL2 at pH 9 and the further incubation for 24 h at 25°C and pH 10, this preparation is 5fold more stable than the optimal BTL2 immobilized on glyoxyl agarose and around 1200fold more stable than the enzyme immobilized on CNBr and further aminated Geobacillus thermocatenulatus

Organism

Organism UniProt Comment Textmining
Geobacillus thermocatenulatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
butyl-Sepharose column chromatography Geobacillus thermocatenulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R,S)-2-O-butyryl-2-phenylacetate + H2O Enantioselectivity of the reaction can be obtained with various methods of enzyme immoblisation and reaction conditions Geobacillus thermocatenulatus (R)-2-O-butyryl-2-phenylacetate + (2S)-hydroxy(phenyl)ethanoic acid + butyrate
-
?
4-nitrophenyl butyrate + H2O
-
Geobacillus thermocatenulatus 4-nitrophenol + butyrate
-
?
diethyl 3-phenylglutarate + H2O
-
Geobacillus thermocatenulatus (S)-ethyl phenylglutarate + (R)-ethyl phenylglutarate + ethanol
-
?

Synonyms

Synonyms Comment Organism
BTL2
-
Geobacillus thermocatenulatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
the optimal preparation retains 100% of its activity after 100 h of incubation at pH 7 and 65°C Geobacillus thermocatenulatus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
the optimal preparation retains 100% of its activity after 100 h of incubation at pH 7 and 65°C Geobacillus thermocatenulatus