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Literature summary for 3.1.1.3 extracted from
- High-level expression of extracellular lipase Lip2 from Yarrowia lipolytica in Pichia pastoris and its purification and characterization (2007), Protein Expr. Purif., 53, 255-263.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene lip2, phylogenetic tree, overexpression in Pichia pastoris strain X-33 using the Saccharomyces cerevisiae secretion signal peptide, alpha-factor, under the control of the methanol inducible promoter of the alcohol oxidase 1 gene, AOX1, the recombinant enzyme is secreted to the culture medium | Yarrowia lipolytica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Yarrowia lipolytica | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 12% sugar content in native and recombinant enzymes, deglycosylation with endoglycosidase H | Yarrowia lipolytica |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme 3.34fold from Pichia pastoris strain X-33 cell culture medium by ultrafiltration and anion exchange chromatography | Yarrowia lipolytica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 19880 | - |
purified extracellular enzyme | Yarrowia lipolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methyl myristate + H2O | best fatty acid methyl ester substrate | Yarrowia lipolytica | methanol + myristate | - |
? |  |
| additional information | the lipase showed high activity toward long-chain fatty acid methyl esters with C12C16, overview | Yarrowia lipolytica | ? | - |
? | |
| olive oil + H2O | best substrate | Yarrowia lipolytica | ? | - |
? | |
| tributyrin + H2O | - |
Yarrowia lipolytica | dibutyrin + butyrate | - |
? | |
| tricaprylin + H2O | - |
Yarrowia lipolytica | dicaprylin + caprylate | - |
? | |
| trilaurin + H2O | low activity | Yarrowia lipolytica | dilaurin + laurate | - |
? | |
| tripalmitin + H2O | low activity | Yarrowia lipolytica | dipalmitin + palmitate | - |
? | |
| tristearin + H2O | low activity | Yarrowia lipolytica | distearin + stearate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| extracellular lipase | - |
Yarrowia lipolytica |
| LIP2 | - |
Yarrowia lipolytica |
| YlLip2 | - |
Yarrowia lipolytica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Yarrowia lipolytica |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 55 | activity range, temperature profile, overview | Yarrowia lipolytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Yarrowia lipolytica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 9 | activity range, pH profile, overview | Yarrowia lipolytica |
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Release 2023.1 (January 2023)
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