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Literature summary for 3.1.1.3 extracted from

  • Kanwar, S.S.; Ghazi, I.A.; Chimni, S.S.; Joshi, G.K.; Rao, G.V.; Kaushal, R.K.; Gupta, R.; Punj, V.
    Purification and properties of a novel extracellular thermotolerant metallolipase of Bacillus coagulans MTCC-6375 isolate (2006), Protein Expr. Purif., 46, 421-428.
    View publication on PubMed
Search for more literature for 3.1.1.3

Inhibitors

Inhibitors Comment Organism Structure
Co2+
-
 Weizmannia coagulans
EDTA reversible by Hg2+ and Al3+ Weizmannia coagulans
Na-molybdate inhibits slightly at 1 mM Weizmannia coagulans
PMSF 98% nhibition at 15 mM Weizmannia coagulans
Zn2+
-
 Weizmannia coagulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
28
-
 4-nitrophenyl palmitate pH 8.5, 45°C Weizmannia coagulans

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Weizmannia coagulans
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ activates at 1 mM Weizmannia coagulans
Ca2+ activates at 1 mM Weizmannia coagulans
Fe3+ activates at 1 mM Weizmannia coagulans
Hg2+ activates at 1 mM Weizmannia coagulans
Mg2+ activates at 1 mM Weizmannia coagulans
additional information enzyme activity is not affected by Na+, K+, and Cu2+ at 1-10 mM Weizmannia coagulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
103000
-
 x * 103000, SDS-PAGE Weizmannia coagulans

Organism

Organism UniProt Comment Textmining
Weizmannia coagulans
-
-
-
Weizmannia coagulans MTCC-6375
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 76.4fold by anion exchange and hydrophobic interaction chromatography to homogeneity Weizmannia coagulans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.98
-
 purified enzyme, substrate 4-nitrophenyl palmitate Weizmannia coagulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl octanoate + H2O
-
 Weizmannia coagulans 4-nitrophenol + octanoate
-
 ?
4-nitrophenyl octanoate + H2O
-
 Weizmannia coagulans MTCC-6375 4-nitrophenol + octanoate
-
 ?
4-nitrophenyl palmitate + H2O
-
 Weizmannia coagulans 4-nitrophenol + palmitate
-
 ?
4-nitrophenyl palmitate + H2O
-
 Weizmannia coagulans MTCC-6375 4-nitrophenol + palmitate
-
 ?

Subunits

Subunits Comment Organism
? x * 103000, SDS-PAGE Weizmannia coagulans

Synonyms

Synonyms Comment Organism
extra-cellular metallolipase
-
 Weizmannia coagulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Weizmannia coagulans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 purified enzyme, 20 min, 25% remaining activity Weizmannia coagulans
35
-
 purified enzyme, 20 min, 63% remaining activity Weizmannia coagulans
45
-
 purified enzyme, 20 min, complete stable Weizmannia coagulans
55
-
 purified enzyme, 20 min, 79% remaining activity Weizmannia coagulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Weizmannia coagulans

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
-
 Weizmannia coagulans