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Literature summary for 3.1.1.3 extracted from
- Metagenomic approach for the isolation of a novel low-temperature-active lipase from uncultured bacteria of marine sediment (2007), FEMS Microbiol. Ecol., 59, 524-534.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| construction and screening of a metagenomic library, DNA and amino acid sequence determination and analysis of ORF and promoter region, overexpression using a copy control fosmid vector in Escherichia coli, the fosmid is isolated using alkaline minipreps according to the protocol for Bacterial Artificial Chromosomes minipreparations | uncultured bacterium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
4-nitrophenyl butyrate | pH 7.2-7.3, 10°C | uncultured bacterium |  |
| 0.32 | - |
4-nitrophenyl butyrate | pH 7.2-7.3, 20°C | uncultured bacterium | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured bacterium | Q3YJM9 | isolated from Baltic Sea soft-bottom sediment samples | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| triacylglycerol + H2O = diacylglycerol + a carboxylate | the enzyme possesses a putative active site, GDSAG, a catalytic triad, formed by Ser148, Glu242 and His272, and a HGG motif | uncultured bacterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-di-O-lauryl-rac-glycero-3-glutaric acid 6'-methylresorufin ester + H2O | chromogenic substrate | uncultured bacterium | ? | - |
? | |
| 4-nitrophenyl caprylate + H2O | - |
uncultured bacterium | 4-nitrophenol + caprylate | - |
? | |
| 4-nitrophenyl decanoate + H2O | - |
uncultured bacterium | 4-nitrophenol + decanoate | - |
? | |
| 4-nitrophenyl laurate + H2O | - |
uncultured bacterium | 4-nitrophenol + laurate | - |
? | |
| 4-nitrophenyl myristate + H2O | - |
uncultured bacterium | 4-nitrophenol + myristate | - |
? | |
| 4-nitrophenylbutyrate + H2O | - |
uncultured bacterium | 4-nitrophenol + butyrate | - |
? | |
| additional information | the lipase hydrolyses 4-nitrophenyl esters of fatty acids with chain lengths up to C14 | uncultured bacterium | ? | - |
? | |
| tributyrin + H2O | - |
uncultured bacterium | dibutyrin + butyrate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| h1Lip1 | - |
uncultured bacterium |
| lipase | - |
uncultured bacterium |
| low-temperature-active lipase | - |
uncultured bacterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
substrate 4-nitrophenyl butyrate | uncultured bacterium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 40 | 33% of maximal activity at 5°C, 44% at 10°C, rapid decrease in activity above 40°C | uncultured bacterium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 40 | h1Lip1 is unstable at 25°C and inactivated at 40°C with t1/2 of below 5 min | uncultured bacterium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 7.3 | assay at | uncultured bacterium |
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