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Literature summary for 3.1.1.3 extracted from
- Exploring the specific features of interfacial enzymology based on lipase studies (2006), Biochim. Biophys. Acta, 1761, 995-1013.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Lipid | the enzyme requires lipids for activity, complete separation of bound lipids from the enzyme leads to extensive loss of activity | Yarrowia lipolytica |  |
| sodium taurodeoxycholate | activates at 2 mM | Yarrowia lipolytica | |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the enzyme can be used in enzyme replacement therapy treating pancreatic exocrine insufficiency | Yarrowia lipolytica |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| enzyme overexpression from genetically modified gene lip2 multicopy strain under control of POX2 promoter | Yarrowia lipolytica |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of strains containing multicopies of gene lip2 | Yarrowia lipolytica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibition of YLLIP2 by bile salts at micellar concentrations | Yarrowia lipolytica |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33385 | - |
x * 38000, SDS-PAGE, x * 36874-38481, four glycosylated isozymes, mass spectrometry, x * 33385, nonglycosylated enzyme, mass spectrometry | Yarrowia lipolytica |
| 38000 | - |
x * 38000, SDS-PAGE, x * 36874-38481, four glycosylated isozymes, mass spectrometry, x * 33385, nonglycosylated enzyme, mass spectrometry | Yarrowia lipolytica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Yarrowia lipolytica | Q9P8F7 | four LIP2 isozymes | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | four isozymes with different glycosylation patterns, overview | Yarrowia lipolytica |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| LIP2 from engineered strains with lip2 multicopy gene by gel filtration and cation exchange chromatography | Yarrowia lipolytica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2200 | - |
purified YLLIP2, substrate soybean oil | Yarrowia lipolytica |
| 10760 | - |
purified YLLIP2, substrate tributyrin | Yarrowia lipolytica |
| 12260 | - |
purified YLLIP2, substrate olive oil | Yarrowia lipolytica |
| 16920 | - |
purified YLLIP2, substrate trioctanoin | Yarrowia lipolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| olive oil + H2O | - |
Yarrowia lipolytica | ? | - |
? | |
| soybean oil + H2O | substrate is stabilized with egg yolk lecithin | Yarrowia lipolytica | ? | - |
? | |
| tributyrin + H2O | - |
Yarrowia lipolytica | ? | - |
? | |
| trioctanoin + H2O | - |
Yarrowia lipolytica | ? | - |
? | |
| triolein + H2O | the enzyme shows a preference for the hydrolysis of the sn-3 ester bond of triolein compared to the sn-1 position | Yarrowia lipolytica | 1,2-diolein + oleate | higher amount of 1,2-diolein | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38000, SDS-PAGE, x * 36874-38481, four glycosylated isozymes, mass spectrometry, x * 33385, nonglycosylated enzyme, mass spectrometry | Yarrowia lipolytica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| fast lipase | - |
Yarrowia lipolytica |
| LIP2 | - |
Yarrowia lipolytica |
| YlLip2 | - |
Yarrowia lipolytica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Yarrowia lipolytica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
pH 6.0, purified enzyme, half-life is more than 4 h | Yarrowia lipolytica |
| 50 | - |
pH 6.0, purified enzyme, half-life is 9 min | Yarrowia lipolytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Yarrowia lipolytica |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
37°C, 1 h, purified enzyme, about 10% remaining activity, complete inactivation after 2 h, half-life 13 min | Yarrowia lipolytica |
| 4 | 8 | 1 h, 37°C, purified enzyme, 74% remaining activity at pH 4.0, 90% at pH 5.0, 98% at pH 6.0, 88% at pH 7.0, and 48% at pH 8.0, half-life at pH 8.0 is 35 min | Yarrowia lipolytica |
| 8.5 | - |
37°C, purified enzyme, complete inactivation after 2 h | Yarrowia lipolytica |
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