Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.3 extracted from

  • Fucinos, P.; Abadin, C.M.; Sanroman, A.; Longo, M.A.; Pastrana, L.; Rua, M.L.
    Identification of extracellular lipases/esterases produced by Thermus thermophilus HB27: Partial purification and preliminary biochemical characterisation (2005), J. Biotechnol., 117, 233-241.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Thermus thermophilus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
x * 34000, SDS-PAGE Thermus thermophilus

Organic Solvent Stability

Organic Solvent Comment Organism
isopropanol 4 h, completely stable in 80% at 30°C Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
-
-

Purification (Commentary)

Purification (Comment) Organism
extracellular enzymes partially over 4.1fold by ammonium sulfate and sodium cholate treatment, ethanol/ether precipitation, and hydrophobic interaction chromatography Thermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.076
-
partially purified enzyme Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl laurate + H2O
-
Thermus thermophilus 4-nitrophenol + laurate
-
?
4-nitrophenyl laurate + H2O
-
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 4-nitrophenol + laurate
-
?

Subunits

Subunits Comment Organism
oligomer x * 34000, SDS-PAGE Thermus thermophilus

Synonyms

Synonyms Comment Organism
BTL2 lipase
-
Thermus thermophilus
lipase
-
Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
Thermus thermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 90
-
Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
partially purified enzyme, stable Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Thermus thermophilus

pH Range

pH Minimum pH Maximum Comment Organism
5 10 and above, activity progressively increases from pH 5.0 to pH 10.0 Thermus thermophilus