Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Pichia pastoris | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
I408D | site-directed mutagenesis, reduced activity, altered substrate specificity, and increased lag time compared to the wild-typ enzyme, water-accessible surface area of the beta5'-loop compared to the wild-type enzyme's loop | Homo sapiens |
I408K | site-directed mutagenesis, reduced activity, altered substrate specificity, and increased lag time compared to the wild-typ enzyme, water-accessible surface area of the beta5'-loop compared to the wild-type enzyme's loop | Homo sapiens |
L412D | site-directed mutagenesis, slightly reduced activity compared to the wild-typ enzyme, water-accessible surface area of the beta5'-loop compared to the wild-type enzyme's loop | Homo sapiens |
L412K | site-directed mutagenesis, slightly reduced activity compared to the wild-typ enzyme, water-accessible surface area of the beta5'-loop compared to the wild-type enzyme's loop | Homo sapiens |
V407D | site-directed mutagenesis, reduced activity, altered substrate specificity, and increased lag time compared to the wild-typ enzyme, water-accessible surface area of the beta5'-loop compared to the wild-type enzyme's loop | Homo sapiens |
V407K | site-directed mutagenesis, reduced activity, altered substrate specificity, and increased lag time compared to the wild-typ enzyme, water-accessible surface area of the beta5'-loop compared to the wild-type enzyme's loop | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of beta5'-loop-mediated interaction of the enzyme with the colipase, overview | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Pichia pastoris | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3900 | - |
purified recombinant wild-type enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Val407 and Ile408 in the beta5'-loop are required for interaction of the enzyme with the colipase in presence of bile salt micelles, overview | Homo sapiens | ? | - |
? | |
tributyrin + H2O | - |
Homo sapiens | dibutyrin + butyrate | - |
? | |
tricaprylin + H2O | - |
Homo sapiens | dicaprylin + caprylate | - |
? | |
triolein + H2O | - |
Homo sapiens | diolein + oleate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure modelling of the beta5'-loop of wild-type and mutant enzymes from crystal structure, PDB code 1LPA | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
pancreatic triglyceride lipase | - |
Homo sapiens |
PTL | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |