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Literature summary for 3.1.1.3 extracted from

  • Deb, C.; Daniel, J.; Sirakova, T.D.; Abomoelak, B.; Dubey, V.S.; Kolattukudy, P.E.
    A novel lipase belonging to the hormone-sensitive lipase family induced under starvation to utilize stored triacylglycerol in Mycobacterium tuberculosis (2006), J. Biol. Chem., 281, 3866-3875.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information starvation induces the enzyme Mycobacterium tuberculosis
Triton X-100 activates at 0.1%, inhibits at higher concentrations Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment) Organism
diverse genes encoding lipases, e.g. gene Rv3097c or lipY, expression of His-tagged LipY and other lipases in in Escherichia coli strain BL21(DE3), LipY in inclusion bodies, overview Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
additional information construction of a gene lipY disruption mutant Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
Mycobacterium tuberculosis
Co2+
-
Mycobacterium tuberculosis
E-600 nearly complete inhibition of recombinant LipY at 0.0005 mM Mycobacterium tuberculosis
Mg2+
-
Mycobacterium tuberculosis
Mn2+
-
Mycobacterium tuberculosis
PMSF 75% inhibition of recombinant LipY at 5 mM Mycobacterium tuberculosis
SDS the complete inhibition is partially reversible by Triton X-100, 20% of maximal activity can be recovered Mycobacterium tuberculosis
Triton X-100 activates at 0.1%, inhibits at higher concentrations Mycobacterium tuberculosis
Zn2+
-
Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.57
-
triolein pH 8.0, 37°C, recombinant LipY Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activates Mycobacterium tuberculosis
Na+ activates Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
x * 45000, recombinant LipY, SDS-PAGE Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P77909 diverse genes encoding lipases with lipY encoding the enzyme with the highest activity, overview
-
Mycobacterium tuberculosis H37Rv P77909 diverse genes encoding lipases with lipY encoding the enzyme with the highest activity, overview
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged LipY, solubilization from inclusion bodies after expression in Escherichia coli strain BL21(DE3), purification by nickel affinity chromatography Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00002 0.0027 crude recombinant lipases in Eschericha coli Mycobacterium tuberculosis
0.041
-
crude recombinant LipY in Eschericha coli Mycobacterium tuberculosis

Storage Stability

Storage Stability Organism
-20°C, recombinant purified LipY, highly stable after 4 cycles of freezing and thawing Mycobacterium tuberculosis
4°C, recombinant purified LipY, 60 days, highly stable Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diolein + H2O
-
Mycobacterium tuberculosis 1-oleoyl-sn-glycerol + oleate
-
?
diolein + H2O
-
Mycobacterium tuberculosis H37Rv 1-oleoyl-sn-glycerol + oleate
-
?
triolein + H2O
-
Mycobacterium tuberculosis diolein + oleate
-
?
triolein + H2O
-
Mycobacterium tuberculosis H37Rv diolein + oleate
-
?

Subunits

Subunits Comment Organism
? x * 45000, recombinant LipY, SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
lipase
-
Mycobacterium tuberculosis
LipY
-
Mycobacterium tuberculosis
More LipY belongs to the HSL family Mycobacterium tuberculosis
TG hydrolase
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mycobacterium tuberculosis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
15 min, recombinant LipY, stable up to, rapid inactivation above Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9 recombinant LipY Mycobacterium tuberculosis

pI Value

Organism Comment pI Value Maximum pI Value
Mycobacterium tuberculosis
-
-
4.5