Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | starvation induces the enzyme | Mycobacterium tuberculosis | |
Triton X-100 | activates at 0.1%, inhibits at higher concentrations | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
diverse genes encoding lipases, e.g. gene Rv3097c or lipY, expression of His-tagged LipY and other lipases in in Escherichia coli strain BL21(DE3), LipY in inclusion bodies, overview | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a gene lipY disruption mutant | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Mycobacterium tuberculosis | |
Co2+ | - |
Mycobacterium tuberculosis | |
E-600 | nearly complete inhibition of recombinant LipY at 0.0005 mM | Mycobacterium tuberculosis | |
Mg2+ | - |
Mycobacterium tuberculosis | |
Mn2+ | - |
Mycobacterium tuberculosis | |
PMSF | 75% inhibition of recombinant LipY at 5 mM | Mycobacterium tuberculosis | |
SDS | the complete inhibition is partially reversible by Triton X-100, 20% of maximal activity can be recovered | Mycobacterium tuberculosis | |
Triton X-100 | activates at 0.1%, inhibits at higher concentrations | Mycobacterium tuberculosis | |
Zn2+ | - |
Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.57 | - |
triolein | pH 8.0, 37°C, recombinant LipY | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | activates | Mycobacterium tuberculosis | |
Na+ | activates | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, recombinant LipY, SDS-PAGE | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P77909 | diverse genes encoding lipases with lipY encoding the enzyme with the highest activity, overview | - |
Mycobacterium tuberculosis H37Rv | P77909 | diverse genes encoding lipases with lipY encoding the enzyme with the highest activity, overview | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged LipY, solubilization from inclusion bodies after expression in Escherichia coli strain BL21(DE3), purification by nickel affinity chromatography | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00002 | 0.0027 | crude recombinant lipases in Eschericha coli | Mycobacterium tuberculosis |
0.041 | - |
crude recombinant LipY in Eschericha coli | Mycobacterium tuberculosis |
Storage Stability | Organism |
---|---|
-20°C, recombinant purified LipY, highly stable after 4 cycles of freezing and thawing | Mycobacterium tuberculosis |
4°C, recombinant purified LipY, 60 days, highly stable | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diolein + H2O | - |
Mycobacterium tuberculosis | 1-oleoyl-sn-glycerol + oleate | - |
? | |
diolein + H2O | - |
Mycobacterium tuberculosis H37Rv | 1-oleoyl-sn-glycerol + oleate | - |
? | |
triolein + H2O | - |
Mycobacterium tuberculosis | diolein + oleate | - |
? | |
triolein + H2O | - |
Mycobacterium tuberculosis H37Rv | diolein + oleate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, recombinant LipY, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Mycobacterium tuberculosis |
LipY | - |
Mycobacterium tuberculosis |
More | LipY belongs to the HSL family | Mycobacterium tuberculosis |
TG hydrolase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mycobacterium tuberculosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
15 min, recombinant LipY, stable up to, rapid inactivation above | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | recombinant LipY | Mycobacterium tuberculosis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
4.5 |