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Literature summary for 3.1.1.3 extracted from

  • Suzuki, T.; Honda, Y.; Mukasa, Y.
    Purification and characterization of lipase in buckwheat seed (2004), J. Agric. Food Chem., 52, 7407-7411.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26500
-
isozyme LIP II, gel filtration Fagopyrum esculentum
28400
-
1 * 28400, isozyme LIP II, SDS-PAGE, 1 * 150000, isozyme LIP I, SDS-PAGE Fagopyrum esculentum
150000
-
1 * 28400, isozyme LIP II, SDS-PAGE, 1 * 150000, isozyme LIP I, SDS-PAGE Fagopyrum esculentum
171000
-
isozyme LIP I, gel filtration Fagopyrum esculentum

Organism

Organism UniProt Comment Textmining
Fagopyrum esculentum
-
var. Kitawasesoba, two isozymes LIP I and LIP II
-

Purification (Commentary)

Purification (Comment) Organism
native isozymes LIP I and LIP II 60fold and 143fold from seed to near homogeneity by ion exchange chromatography and gel filtration, respectively Fagopyrum esculentum

Source Tissue

Source Tissue Comment Organism Textmining
embryo high activity Fagopyrum esculentum
-
endosperm low activity compare to the embryo Fagopyrum esculentum
-
testis very low activity Fagopyrum esculentum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.11
-
purified isozyme LIP I, substrate triolein, 30°C Fagopyrum esculentum
0.36
-
purified isozyme LIP I, substrate 4-nitrophenyl laurate, 22°C Fagopyrum esculentum
0.73
-
purified isozyme LIP II, substrate triolein, 30°C Fagopyrum esculentum
0.86
-
purified isozyme LIP II, substrate 4-nitrophenyl laurate, 22°C Fagopyrum esculentum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
Fagopyrum esculentum 4-nitrophenol + acetate
-
?
4-nitrophenyl butyrate + H2O
-
Fagopyrum esculentum 4-nitrophenol + butyrate
-
?
4-nitrophenyl caproate + H2O
-
Fagopyrum esculentum 4-nitrophenol + caproate
-
?
4-nitrophenyl laurate + H2O best substrate Fagopyrum esculentum 4-nitrophenol + laurate
-
?
olein + H2O low activity with isozyme LIP II, residual activity with isozyme LIP I Fagopyrum esculentum glycerol + oleate
-
?
palmitin + H2O low activity with isozyme LIP II, residual activity with isozyme LIP I Fagopyrum esculentum glycerol + palmitate
-
?
triolein + H2O good substrate Fagopyrum esculentum diolein + oleate
-
?
tripalmitin + H2O low activity with isozyme LIP II, residual activity with isozyme LIP I Fagopyrum esculentum dipalmitin + palmitate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 28400, isozyme LIP II, SDS-PAGE, 1 * 150000, isozyme LIP I, SDS-PAGE Fagopyrum esculentum

Synonyms

Synonyms Comment Organism
LIP
-
Fagopyrum esculentum
lipase
-
Fagopyrum esculentum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
isozyme LIP I, and isozyme LIP I with substrate triolein Fagopyrum esculentum
40
-
isozyme LIP I with substrate 4-nitrophenyl laurate Fagopyrum esculentum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 70 isozymes LIP I amd II, activity is dependent on substrate, fully stable at 10°C and 45% remaining activity at 70°C with substrate triolein after 4 h Fagopyrum esculentum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
isozyme LIP I with substrate triolein Fagopyrum esculentum
5
-
isozyme LIP I with substrate 4-nitrophenyl laurate Fagopyrum esculentum
6
-
isozyme LIP II Fagopyrum esculentum

pH Range

pH Minimum pH Maximum Comment Organism
3 8 isozymes LIP I and II with substrate 4-nitrophenyl laurate Fagopyrum esculentum
3 7 isozymes LIP I and II with substrate triolein Fagopyrum esculentum