Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyl 4-nitrophenyl phosphate | rapid and complete inhibition | Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Pseudomonas aeruginosa | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas aeruginosa PAC1R | - |
- |
- |
Purification (Comment) | Organism |
---|---|
extracellular enzyme by chromatographical isoelectric focusing | Pseudomonas aeruginosa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
triacylglycerol + H2O = diacylglycerol + a carboxylate | the enzyme contains an active site serine residue | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl palmitate + H2O | - |
Pseudomonas aeruginosa | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl palmitate + H2O | - |
Pseudomonas aeruginosa PAC1R | 4-nitrophenol + palmitate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme contains the conserved N-terminal consensus sequence KYP-IVL-VHG, sodium deoxycholate and cetyltrimethylammonium bromide shift the electrophoretic mobility of the enzyme in anodal or cathodal direction, respectively, amphiphilic enzyme | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
extracellular lipase | - |
Pseudomonas aeruginosa |
lipase | - |
Pseudomonas aeruginosa |
triacylglycerol acylhydrolase | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Pseudomonas aeruginosa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas aeruginosa | isoelectric focusing | - |
5.7 |