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Literature summary for 3.1.1.3 extracted from
- Influence of the conformational flexibility on the kinetics and dimerization process of two Candida rugosa lipase isoenzymes (2001), FEBS Lett., 501, 87-91.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| sodium deoxycholate | isozymes Lip3 and Lip1, inhibition kinetics | Diutina rugosa |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Diutina rugosa |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
monomeric Lip3, gel filtration | Diutina rugosa |
| 60000 | - |
1 * 60000, Lip3, SDS-PAGE | Diutina rugosa |
| 60000 | - |
2 * 60000, Lip3, SDS-PAGE | Diutina rugosa |
| 117000 | - |
dimeric Lip3, gel filtration | Diutina rugosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Diutina rugosa | - |
2 isoenzymes Lip1 and Lip3 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 910 | - |
purified Lip3 monomer | Diutina rugosa |
| 1030 | - |
purified Lip3 dimer | Diutina rugosa |
| 1500 | - |
purified Lip1 dimer | Diutina rugosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| triacetin + H2O | - |
Diutina rugosa | diacetin + acetic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 60000, Lip3, SDS-PAGE | Diutina rugosa |
| monomer | 1 * 60000, Lip3, SDS-PAGE | Diutina rugosa |
| More | study of dimerisation process of lipase isozymes, isozyme Lip3 exists in a dimeric and a monomeric form | Diutina rugosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipase | - |
Diutina rugosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Diutina rugosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Diutina rugosa |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Diutina rugosa | Lip3 monomer, isoelectric focusing | - |
5.8 |
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