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Literature summary for 3.1.1.3 extracted from

  • Miled, N.; Bussetta, C.; De Caro, A.; Riviere, M.; Berti, L.; Canaan, S.
    Importance of the lid and cap domains for the catalytic activity of gastric lipases (2003), Comp. Biochem. Physiol. B, 136B, 131-138.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type enzyme and mutants lacking the cap and the lid domain via baculovirus transfection in insect cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of mutants lacking the cap and the lid domain by oligonucleotide-directed mutagenesis, influencing the expresion level, stability, activity, and reaction kinetics Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secretion in the stomach Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
x * 47000, wild-type enzyme, SDS-PAGE, x * 43000, lid domain lacking mutant, SDS-PAGE, x * 32000, cap domain lacking mutant, SDS-PAGE Homo sapiens
43000
-
x * 47000, wild-type enzyme, SDS-PAGE, x * 43000, lid domain lacking mutant, SDS-PAGE, x * 32000, cap domain lacking mutant, SDS-PAGE Homo sapiens
47000
-
x * 47000, wild-type enzyme, SDS-PAGE, x * 43000, lid domain lacking mutant, SDS-PAGE, x * 32000, cap domain lacking mutant, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
gastric lipase
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from insect cells, mutant lacking the cap domain is only stable at acidic pH value during purification Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
triacylglycerol + H2O = diacylglycerol + a carboxylate catalytic serine residue, deeply buried under a domain called the extrusion domain, which is composed of a cap and a lid segmentof 58 amino acids, catalytic reaction mechanism Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
stomach
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.08
-
purified recombinant mutant enzyme lacking the cap domain, substrate triolein Homo sapiens
0.5
-
purified recombinant mutant enzyme lacking the lid domain, substrate triolein Homo sapiens
30
-
purified recombinant wild-type enzyme, substrate triolein Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
triolein + H2O
-
Homo sapiens diolein + oleate
-
?

Subunits

Subunits Comment Organism
? x * 47000, wild-type enzyme, SDS-PAGE, x * 43000, lid domain lacking mutant, SDS-PAGE, x * 32000, cap domain lacking mutant, SDS-PAGE Homo sapiens
More epitope mapping Homo sapiens

Synonyms

Synonyms Comment Organism
HGL
-
Homo sapiens
More the enzyme belongs to the alpha/beta hydrolase fold family Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
assay at Homo sapiens