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Literature summary for 3.1.1.3 extracted from

  • Rathi, P.; Bradoo, S.; Saxena, R.K.; Gupta, R.
    A hyper-thermostable, alkaline lipase from Pseudomonas sp. with the property of thermal activation (2000), Biotechnol. Lett., 22, 495-498.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
conatians a hyper-thermostable lipase
-

Purification (Commentary)

Purification (Comment) Organism
partial, 6.8fold Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
167
-
partially purified enzyme Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
castor oil + H2O major acyl group is ricinoleic acid Pseudomonas sp. ?
-
?
coconut oil + H2O major acyl group is palmitic acid Pseudomonas sp. ?
-
?
corn oil + H2O major acyl groups are oleic acid and linoleic acid Pseudomonas sp. ?
-
?
groundnut oil + H2O major acyl group is oleic acid Pseudomonas sp. ?
-
?
linseed oil + H2O major acyl groups are oleic and linoleic acid Pseudomonas sp. ?
-
?
mustard oil + H2O major acyl groups are erucic and oleic acid Pseudomonas sp. ?
-
?
neem oil + H2O major acyl groups are palmitic, stearic and oleic acid Pseudomonas sp. ?
-
?
olive oil + H2O best substrate, major acyl group is oleic acid Pseudomonas sp. ?
-
?
soybean oil + H2O major acyl groups are oleic acid and linoleic acid Pseudomonas sp. ?
-
?
sunflower oil + H2O major acyl group is linoleic acid Pseudomonas sp. ?
-
?

Synonyms

Synonyms Comment Organism
lipase
-
Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
8 h 100% stable, 13 h loss of 50% activity, after 2 h at 90°C 30% activation is observed Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
11
-
-
Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
9 12
-
Pseudomonas sp.