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Literature summary for 3.1.1.3 extracted from
- Thermostable lipase of Bacillus stearothermophilus: high-level production, purification, and calcium-dependent thermostability (2000), Biosci. Biotechnol. Biochem., 64, 280-286.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| high-level expression in Escherichia coli BL21(DE3) | Geobacillus stearothermophilus |
General Stability
| General Stability | Organism |
|---|---|
| Ca2+ ions stabilize the tertiary structure of the enzyme | Geobacillus stearothermophilus |
| Triton X-100 destabilizes the purified enzyme during storage at -20°C and -70°C | Geobacillus stearothermophilus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required for thermostability | Geobacillus stearothermophilus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
gel filtration | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
| Geobacillus stearothermophilus L1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant from Escherichia coli, to homogeneity | Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1690 | - |
purifed enzyme | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | substrate is olive oil emulsion | Geobacillus stearothermophilus | ? | - |
? | |
| additional information | substrate is olive oil emulsion | Geobacillus stearothermophilus L1 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L1 lipase | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 68 | - |
with olive oil as substrate | Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermostability is increased by 8-10 degrees in presence of Ca2+ | Geobacillus stearothermophilus |
| 55 | - |
30 min, thermostable enzyme | Geobacillus stearothermophilus |
| 70 | - |
inactivation within 13 min | Geobacillus stearothermophilus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Geobacillus stearothermophilus | isoelectric fosucing | - |
7.4 |
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Release 2023.1 (January 2023)
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