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Literature summary for 3.1.1.3 extracted from

  • Sayari, A.; Agrebi, N.; Jaoua, S.; Gargouri, Y.
    Biochemical and molecular characterization of Staphylococcus simulans lipase (2001), Biochimie, 83, 863-871.
    View publication on PubMed
Search for more literature for 3.1.1.3

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination, analysis, expression in Escherichia coli DH5alpha Staphylococcus simulans

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by bile salts or amphiphilic proteins Staphylococcus simulans

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular enzyme is secreted to the culture medium Staphylococcus simulans
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates 1.6fold, not required for expression of the enzyme, optimal at 3 mM Staphylococcus simulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 4 * 45000, SDS-PAGE Staphylococcus simulans
158000
-
 gel filtration Staphylococcus simulans

Organism

Organism UniProt Comment Textmining
Staphylococcus simulans Q84EK3
-
-

Purification (Commentary)

Purification (Comment) Organism
397fold, to homogeneity from culture supernatant Staphylococcus simulans

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1100
-
 purified enzyme, substrate tributyrin, 3 mM CaCl2 Staphylococcus simulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme hydrolyzes triacylglycerols without chain length specificity Staphylococcus simulans ?
-
 ?
tributyrin + H2O
-
 Staphylococcus simulans dibutyrin + butyrate
-
 ?
triolein + H2O
-
 Staphylococcus simulans diolein + oleate
-
 ?
tripropionin + H2O
-
 Staphylococcus simulans dipropionin + propionate
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 45000, SDS-PAGE Staphylococcus simulans

Synonyms

Synonyms Comment Organism
lipase
-
 Staphylococcus simulans
SSL
-
 Staphylococcus simulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Staphylococcus simulans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 inactivation after a few min Staphylococcus simulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Staphylococcus simulans