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Literature summary for 3.1.1.3 extracted from
- Expression and characterization of Ca2+-independent lipase from Bacillus pumilus B26 (2002), Biochim. Biophys. Acta, 1583, 205-212.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA sequence determination and analysis, expression in Escherichia coli XL1-Blue and in Bacillus subtilis DB104 | Bacillus pumilus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | secretion to the culture medium | Bacillus pumilus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | enzyme shows Ca2+-independent thermostability and catalytic activity, enzyme contains no Ca2+-binding motif around the catalytic His156 residue | Bacillus pumilus |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus pumilus | - |
Ca2+-independent lipase | - |
| Bacillus pumilus B26 | - |
Ca2+-independent lipase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Bacillus subtilis culture supernatant, 4.3fold, purification of the wild-type Bacillus pumilus strain is not possible due to the low expression level | Bacillus pumilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 138 | - |
purified enzyme, substrate is olive oil emulsion | Bacillus pumilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl butyrate + H2O | the enzyme shows a typical interfacial activation mechanism towards the substrate | Bacillus pumilus | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl butyrate + H2O | the enzyme shows a typical interfacial activation mechanism towards the substrate | Bacillus pumilus B26 | 4-nitrophenol + butyrate | - |
? | |
| additional information | an emulsion containing olive oil and gum arabic is used as substrate, enzyme shows a preference for short-chain triacylglycerides | Bacillus pumilus | ? | - |
? | |
| additional information | an emulsion containing olive oil and gum arabic is used as substrate, enzyme shows a preference for short-chain triacylglycerides | Bacillus pumilus B26 | ? | - |
? | |
| tributyrin + H2O | - |
Bacillus pumilus | dibutyrin + butyrate | - |
? | |
| tributyrin + H2O | - |
Bacillus pumilus B26 | dibutyrin + butyrate | - |
? | |
| triolein + H2O | low activity | Bacillus pumilus | diolein + oleate | - |
? | |
| triolein + H2O | low activity | Bacillus pumilus B26 | diolein + oleate | - |
? | |
| tripropionin + H2O | the enzyme shows a typical interfacial activation mechanism towarsd the substrate | Bacillus pumilus | dipropionin + propionate | - |
? | |
| tripropionin + H2O | the enzyme shows a typical interfacial activation mechanism towarsd the substrate | Bacillus pumilus B26 | dipropionin + propionate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipase B26 | - |
Bacillus pumilus |
| More | the enzyme belongs to the lipase family I.4 | Bacillus pumilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Bacillus pumilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
stable up to, in presence or absence of 10 mM Ca2+ | Bacillus pumilus |
| 45 | - |
rapid decrease in activity | Bacillus pumilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Bacillus pumilus |
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