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Literature summary for 3.1.1.20 extracted from

  • Niehaus, J.U.; Gross, G.G.
    A gallotannin degrading esterase from leaves of pedunculate oak (1997), Phytochemistry, 45, 1555-1560.
No PubMed abstract available
Search for more literature for 3.1.1.20

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.85
-
 1-O-syringoyl-beta-D-glucose
-
 Quercus robur
0.99
-
 m-digallic acid
-
 Quercus robur
1.3
-
 1,6-di-O-galloyl-beta-D-glucose
-
 Quercus robur
2
-
 1-O-benzoyl-beta-D-glucose
-
 Quercus robur
2.1
-
 1-O-p-hydroxybenzoyl-beta-D-glucose
-
 Quercus robur
2.3
-
 ethyl gallate
-
 Quercus robur
2.3
-
 1,2,6-Tri-O-galloyl-beta-D-glucose
-
 Quercus robur
2.4
-
 propyl gallate
-
 Quercus robur
4
-
 1-O-Veratroyl-beta-D-glucose
-
 Quercus robur
4.1
-
 chlorogenic acid
-
 Quercus robur
4.7
-
 6-O-galloyl-beta-D-glucose
-
 Quercus robur
5.5
-
 3,6-di-O-galloyl-beta-D-glucose
-
 Quercus robur
5.9
-
 1,2,3,4,6-pentagalloyl glucose
-
 Quercus robur
6
-
 1-O-Protocatechuoyl-beta-D-glucose
-
 Quercus robur
6
-
 4-O-meta-digalloyl-1,2,3,6-tetra-O-galloyl beta-D-glucose
-
 Quercus robur
6.9
-
 1,2,3,6-tetra-O-galloyl-beta-D-glucose
-
 Quercus robur
9.1
-
 1-O-Galloyl-beta-D-glucose
-
 Quercus robur
12.7
-
 1-O-anisoyl-beta-D-glucose
-
 Quercus robur
17.5
-
 1-O-vanilloyl-beta-D-glucose
-
 Quercus robur
71.4
-
 methyl gallate
-
 Quercus robur

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 2 * 75000, SDS-PAGE, can exist as dimer or tetramer Quercus robur
75000
-
 4 * 75000, SDS-PAGE, can exist as dimer or tetramer Quercus robur
150000
-
 can exist as tetramer or dimer, gel fitration Quercus robur
300000
-
 can exist as tetramer or dimer, gel fitration Quercus robur

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tannic acid + H2O Quercus robur
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Quercus robur
-
 Qercus pedunculata
-

Purification (Commentary)

Purification (Comment) Organism
1910fold to apparent homogeneity Quercus robur

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.1602
-
 6 step purified enzyme Quercus robur

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2,3,4,6-pentagalloyl glucose + H2O
-
 Quercus robur gallic acid + D-glucose
-
 ?
1,2,3,6-tetra-O-galloyl-beta-D-glucose + H2O
-
 Quercus robur ?
-
 ?
1,2,6-tri-O-galloyl-beta-D-glucose + H2O
-
 Quercus robur ?
-
 ?
1,6-di-O-galloyl-beta-D-glucose + H2O
-
 Quercus robur ?
-
 ?
1-O-anisoyl-beta-D-glucose + H2O
-
 Quercus robur anisic acid + glucose
-
 ?
1-O-benzoyl-beta-D-glucose + H2O
-
 Quercus robur benzoic acid + beta-D-glucose
-
 ?
1-O-galloyl-beta-D-glucose + H2O
-
 Quercus robur gallic acid + beta-D-glucose
-
 ?
1-O-p-hydroxybenzoyl-beta-D-glucose + H2O
-
 Quercus robur 4-hydroxybenzoate + beta-D-glucose
-
 ?
1-O-protocatechuoyl-beta-D-glucose + H2O
-
 Quercus robur protocatechuate + glucose
-
 ?
1-O-syringoyl-beta-D-glucose + H2O
-
 Quercus robur 4-hydroxy-3,5-dimethoxy benzoic acid + beta-D-glucose
-
 ?
1-O-vanilloyl-beta-D-glucose + H2O
-
 Quercus robur 4-hydroxy-3-methoxy benzoic acid + beta-D-glucose
-
 ?
1-O-veratroyl-beta-D-glucose + H2O
-
 Quercus robur 3,4-dimethoxybenzoic acid + beta-D-glucose
-
 ?
3,6-di-O-galloyl-beta-D-glucose + H2O
-
 Quercus robur ?
-
 ?
4-O-meta-digalloyl-1,2,3,6-tetra-O-galloyl beta-D-glucose + H2O
-
 Quercus robur ?
-
 ?
6-O-galloyl-beta-D-glucose + H2O
-
 Quercus robur gallic acid + beta-D-glucose
-
 ?
chlorogenic acid + H2O
-
 Quercus robur ?
-
 ?
ethyl gallate + H2O
-
 Quercus robur gallic acid + ethanol
-
 ?
m-digallic acid
-
 Quercus robur 2 gallate
-
 ?
meta-digallic acid + H2O
-
 Quercus robur gallic acid
-
 ?
methyl gallate + H2O
-
 Quercus robur gallic acid + methanol
-
 ?
n-propyl gallate + H2O
-
 Quercus robur gallic acid + n-propanol
-
 ?
tannic acid + H2O
-
 Quercus robur gallic acid + ?
-
 ?
tannic acid + H2O
-
 Quercus robur ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 75000, SDS-PAGE, can exist as dimer or tetramer Quercus robur
tetramer 4 * 75000, SDS-PAGE, can exist as dimer or tetramer Quercus robur

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40 activation energy of 24.3 kJ per mol Quercus robur

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
-
 42% of the maximal activity Quercus robur
50
-
 heat-denaturation above Quercus robur
70 80 complete inactivation Quercus robur

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3 5 potassium phosphate/citrate buffer Quercus robur

pH Range

pH Minimum pH Maximum Comment Organism
4 6.3 half-maximal acivities at pH 4.0 and pH 6.3, inactive below pH 3.8 and above pH 7.8 Quercus robur

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 greatest stability after preincubation at 30°C for 60 min or 24 h Quercus robur