Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DMSO | 144% activity at 1 mM | Streptococcus gallolyticus | |
Tween 80 | 119% activity at 1 mM | Streptococcus gallolyticus |
Application | Comment | Organism |
---|---|---|
degradation | TanSg1 is a tannase with potential industrial interest regarding the biodegradation of tannin waste or its bioconversion into biologically active products | Streptococcus gallolyticus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptococcus gallolyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 16% residual activity at 1 mM | Streptococcus gallolyticus | |
EDTA | 80% residual activity at 1 mM | Streptococcus gallolyticus | |
Hg2+ | 6% residual activity at 1 mM | Streptococcus gallolyticus | |
K+ | 69% residual activity at 1 mM | Streptococcus gallolyticus | |
Mg2+ | 65% residual activity at 1 mM | Streptococcus gallolyticus | |
Triton X-100 | 78% residual activity at 1 mM | Streptococcus gallolyticus | |
Urea | 35% residual activity at 1 mM | Streptococcus gallolyticus | |
Zn2+ | 10% residual activity at 1 mM | Streptococcus gallolyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 120% activity at 1 mM | Streptococcus gallolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52980 | - |
x * 52980, calculated from amino acid sequence | Streptococcus gallolyticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
gallotannin + H2O | Streptococcus gallolyticus | - |
gallate + D-glucose + ? | - |
? | |
tannic acid + H2O | Streptococcus gallolyticus | - |
gallate + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus gallolyticus | - |
- |
- |
Streptococcus gallolyticus UCN34 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Talon Superflow resin column chromatography | Streptococcus gallolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butyl 4-hydroxybenzoate + H2O | - |
Streptococcus gallolyticus | butanol + 4-hydroxybenzoate | - |
? | |
epigallocatechin gallate + H2O | - |
Streptococcus gallolyticus | epigallocatechin + gallate | - |
? | |
ethyl 3,4-dihydroxybenzoate + H2O | - |
Streptococcus gallolyticus | ethanol + 3,4-dihydroxybenzoate | - |
? | |
ethyl 3,4-dihydroxybenzoate + H2O | - |
Streptococcus gallolyticus UCN34 | ethanol + 3,4-dihydroxybenzoate | - |
? | |
ethyl 3,5-dihydroxybenzoate + H2O | - |
Streptococcus gallolyticus | ethanol + 3,5-dihydroxybenzoate | - |
? | |
ethyl 4-hydroxybenzoate + H2O | - |
Streptococcus gallolyticus | ethanol + 4-hydroxybenzoate | - |
? | |
ethyl 4-hydroxybenzoate + H2O | - |
Streptococcus gallolyticus UCN34 | ethanol + 4-hydroxybenzoate | - |
? | |
ethyl benzoate + H2O | - |
Streptococcus gallolyticus | ethanol + benzoate | - |
? | |
ethyl benzoate + H2O | - |
Streptococcus gallolyticus UCN34 | ethanol + benzoate | - |
? | |
ethyl gallate + H2O | - |
Streptococcus gallolyticus | gallate + ethanol | - |
? | |
ferulic ethyl ester + H2O | - |
Streptococcus gallolyticus | ethanol + ferulate | - |
? | |
ferulic methyl ester + H2O | - |
Streptococcus gallolyticus | methanol + ferulate | - |
? | |
gallotannin + H2O | - |
Streptococcus gallolyticus | gallate + D-glucose + ? | - |
? | |
lauryl gallate + H2O | - |
Streptococcus gallolyticus | gallate + lauric acid | - |
? | |
methyl 2,4-dihydroxybenzoate + H2O | - |
Streptococcus gallolyticus | methanol + 2,4-dihydroxybenzoate | - |
? | |
methyl 4-hydroxybenzoate + H2O | - |
Streptococcus gallolyticus | methanol + 4-hydroxybenzoate | - |
? | |
methyl benzoate + H2O | - |
Streptococcus gallolyticus | methanol + benzoate | - |
? | |
methyl benzoate + H2O | - |
Streptococcus gallolyticus UCN34 | methanol + benzoate | - |
? | |
methyl gallate + H2O | - |
Streptococcus gallolyticus | gallate + methanol | - |
? | |
methyl gentisate + H2O | - |
Streptococcus gallolyticus | methanol + gentisate | - |
? | |
methyl salicylate + H2O | - |
Streptococcus gallolyticus | methanol + salicylate | - |
? | |
methyl vanillate + H2O | - |
Streptococcus gallolyticus | methanol + vanillate | - |
? | |
methyl vanillate + H2O | - |
Streptococcus gallolyticus UCN34 | methanol + vanillate | - |
? | |
propyl 4-hydroxybenzoate + H2O | - |
Streptococcus gallolyticus | propanol + 4-hydroxybenzoate | - |
? | |
propyl gallate + H2O | - |
Streptococcus gallolyticus | gallate + propanol | - |
? | |
protocatechuic acid ethyl ester + H2O | - |
Streptococcus gallolyticus | ethanol + protocatechuate | - |
? | |
tannic acid + H2O | - |
Streptococcus gallolyticus | gallate + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52980, calculated from amino acid sequence | Streptococcus gallolyticus |
Synonyms | Comment | Organism |
---|---|---|
GALLO_1609 | locus name | Streptococcus gallolyticus |
tannin acyl hydrolase | - |
Streptococcus gallolyticus |
TanSg1 | - |
Streptococcus gallolyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Streptococcus gallolyticus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 65 | active between 4 and 65°C | Streptococcus gallolyticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 65 | the protein dramatically loses its activity after 30 min at 65°C. However, the enzyme keeps more than 70% activity after 30 min of incubation at 55°C. The enzyme shows about 50 and 25% of the maximal activity after 6 and 18 h of incubation at 45°C, respectively | Streptococcus gallolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Streptococcus gallolyticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | highly active at pH 6.0-8.0. At pH 5.0, the enzyme shows less than 20% activity. At pH 3.0 and 10.0, the enzyme completely loses its activity | Streptococcus gallolyticus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Streptococcus gallolyticus | calculated from amino acid sequence | - |
5.1 |