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Literature summary for 3.1.1.20 extracted from

  • Sharma, K.; John, P.
    Purification and characterization of tannase and tannase gene from Enterobacter sp. (2011), Process Biochem., 46, 240-244.
No PubMed abstract available
Search for more literature for 3.1.1.20

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7
-
 methyl gallate pH and temperature not specified in the publication Enterobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 x * 90000, SDS-PAGE Enterobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tannic acid + H2O Enterobacter sp.
-
 gallic acid + D-glucose
-
 ?
tannic acid + H2O Enterobacter sp. KPJ03
-
 gallic acid + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Enterobacter sp. C7EDT0
-
-
Enterobacter sp. KPJ03 C7EDT0
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE cellulose column chromatography, and Sephadex G-100 gel filtration Enterobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.084
-
 crude extract, pH and temperature not specified in the publication Enterobacter sp.
13.63
-
 after 162fold purification, pH and temperature not specified in the publication Enterobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl gallate + H2O
-
 Enterobacter sp. gallate + methanol
-
 ?
methyl gallate + H2O
-
 Enterobacter sp. KPJ03 gallate + methanol
-
 ?
tannic acid + H2O
-
 Enterobacter sp. gallic acid + D-glucose
-
 ?
tannic acid + H2O
-
 Enterobacter sp. KPJ03 gallic acid + D-glucose
-
 ?

Subunits

Subunits Comment Organism
? x * 90000, SDS-PAGE Enterobacter sp.

Synonyms

Synonyms Comment Organism
tannin acyl hydrolase
-
 Enterobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Enterobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 the enzyme retains 20% of its activity at 60°C Enterobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Enterobacter sp.

General Information

General Information Comment Organism
physiological function tannase and the organism itself are employed to protect grazing animals and environment against the toxic effects caused by tannins in them Enterobacter sp.