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Literature summary for 3.1.1.20 extracted from

  • Beena, P.S.; Soorej, M.B.; Elyas, K.K.; Sarita, G.B.; Chandrasekaran, M.
    Acidophilic tannase from marine Aspergillus awamori BTMFW032 (2010), J. Microbiol. Biotechnol., 20, 1403-1414.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol enhances activity at low concentrations of 0.2% (v/v) (108.1% residual activity) to 0.6% (v/v) (111.8% residual activity) Aspergillus awamori
Sodium thioglycolate an increase in residual enzyme activity from 23.1% (0.2% concentration) to a maximum of 321.2% (1.0% concentration) is noted along with an increase in its concentration Aspergillus awamori

Inhibitors

Inhibitors Comment Organism Structure
Al3+ complete inhibition at 1 mM Aspergillus awamori
Ba2+ complete inhibition at 5 mM Aspergillus awamori
beta-mercaptoethanol
-
Aspergillus awamori
Ca2+ 0.84% residual activity at 20 mM Aspergillus awamori
Cd2+ 11.82% residual activity at 20 mM Aspergillus awamori
Co2+ 39.06% residual activity at 20 mM Aspergillus awamori
Cu2+ 10.58% residual activity at 20 mM Aspergillus awamori
dithiothreitol displays inhibitory properties at higher concentrations of 1.0% to 5.0% (v/v) Aspergillus awamori
H2O2 H2O2 concentrations of less than 2% and higher than 10% lead to a decline in enzyme activity Aspergillus awamori
Hg2+ 53.02% residual activity at 20 mM Aspergillus awamori
Li+ 3.07% residual activity at 20 mM Aspergillus awamori
Mg2+ 7.03% residual activity at 20 mM Aspergillus awamori
Mn2+ 10.18% residual activity at 20 mM Aspergillus awamori
Mo2+ 22.48% residual activity at 20 mM Aspergillus awamori
Zn2+ 30.54% residual activity at 20 mM Aspergillus awamori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.9
-
methyl gallate at pH 2.0, 30°C Aspergillus awamori

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Aspergillus awamori
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe3+ 144.44% activity at 10 mM Aspergillus awamori
K+ 176.62% activity at 10 mM Aspergillus awamori
Na+ 120.37% activity at 20 mM Aspergillus awamori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37800
-
6 * 37800, SDS-PAGE Aspergillus awamori
230000
-
gel filtration Aspergillus awamori

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone about 70% activity after 1 h at 10% (v/v) Aspergillus awamori
acetonitrile about 85% activity after 1 h at 10% (v/v) Aspergillus awamori
benzene about 45% activity after 1 h at 10% (v/v) Aspergillus awamori
Brij-35 the enzyme is not stable in the presence of Brij-35, with less than 82% residual activity at 0.1-1% (v/v) Aspergillus awamori
Butanol about 70% activity after 1 h at 10% (v/v) Aspergillus awamori
chloroform about 60% activity after 1 h at 10% (v/v) Aspergillus awamori
DMSO at a 60% concentration of DMSO, the enzyme retains a 61% activity after 24 h Aspergillus awamori
Ethanol about 80% activity after 1 h at 10% (v/v) Aspergillus awamori
hexane about 80% activity after 1 h at 10% (v/v) Aspergillus awamori
isopropanol about 70% activity after 1 h at 10% (v/v) Aspergillus awamori
Methanol about 80% activity after 1 h at 10% (v/v) Aspergillus awamori
Triton X-100 at high concentrations of between 0.6% (v/v) (79.06% residual activity) to 1.0% (v/v) (54.26% residual activity), lead to a loss of enzyme stability, although the enzyme is stable at a 0.2% (v/v) concentration (112.2% residual activity) with an enhanced enzyme activity at a 0.4% concentration (171.36% residual activity) Aspergillus awamori
Tween 80 Tween 80 leads to an enhanced enzyme activity at up to a 0.6% (v/v) concentration (157.89% residual activity), but displays inhibitory properties at a high concentration of 1.0% (v/v) (83.5% residual activity) Aspergillus awamori

Organism

Organism UniProt Comment Textmining
Aspergillus awamori C7F6Y1
-
-
Aspergillus awamori BTMFW032 C7F6Y1
-
-

Oxidation Stability

Oxidation Stability Organism
increases in hydrogen peroxide concentrations result in enhanced enzyme activity in the range of 2% (161.1% activity) to 10% (220% activity) Aspergillus awamori

Purification (Commentary)

Purification (Comment) Organism
ultrafiltration and Sephadex G-200 gel filtration Aspergillus awamori

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
436.7
-
crude extract, pH 2.0, 30°C Aspergillus awamori
2762
-
after 6.32fold purification, pH 2.0, 30°C Aspergillus awamori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl gallate + H2O best substrate Aspergillus awamori gallate + methanol
-
?
methyl gallate + H2O best substrate Aspergillus awamori BTMFW032 gallate + methanol
-
?
propyl gallate + H2O
-
Aspergillus awamori gallate + propanol
-
?
propyl gallate + H2O
-
Aspergillus awamori BTMFW032 gallate + propanol
-
?
tannic acid + H2O lowest activity Aspergillus awamori gallic acid + D-glucose
-
?
tannic acid + H2O lowest activity Aspergillus awamori BTMFW032 gallic acid + D-glucose
-
?

Subunits

Subunits Comment Organism
homohexamer 6 * 37800, SDS-PAGE Aspergillus awamori

Synonyms

Synonyms Comment Organism
tannin acyl hydrolase
-
Aspergillus awamori

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Aspergillus awamori

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 80 the enzyme activity declines markedly along with increases in temperature above 60°C Aspergillus awamori

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 70 the enzyme is stable only at 30°C for 24 h, retaining about 60% activity. The residual activity is 72% at 40°C for 2 h, 54% at 50°C for 6 h, 53% at 60°C for 4 h, and 72% at 70°C for 1 h Aspergillus awamori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2
-
two pH optima, pH 2.0 and pH 8.0, are recorded Aspergillus awamori
8
-
two pH optima, pH 2.0 and pH 8.0, are recorded Aspergillus awamori

pH Range

pH Minimum pH Maximum Comment Organism
1 7 the enzyme records a significant level of activity even at a pH of 1.0, however, the enzyme activity declines rapidly at pH 3.0-4.0 and is almost nil at a pH of 7.0 Aspergillus awamori

pH Stability

pH Stability pH Stability Maximum Comment Organism
2
-
the enzyme is stable (99%) over a relatively long duration of incubation (up to 24 h) only at pH 2.0. At other pH levels, the enzyme is not stable Aspergillus awamori

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus awamori isoelectric focusing
-
4.4