Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | enhances activity at low concentrations of 0.2% (v/v) (108.1% residual activity) to 0.6% (v/v) (111.8% residual activity) | Aspergillus awamori | |
Sodium thioglycolate | an increase in residual enzyme activity from 23.1% (0.2% concentration) to a maximum of 321.2% (1.0% concentration) is noted along with an increase in its concentration | Aspergillus awamori |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | complete inhibition at 1 mM | Aspergillus awamori | |
Ba2+ | complete inhibition at 5 mM | Aspergillus awamori | |
beta-mercaptoethanol | - |
Aspergillus awamori | |
Ca2+ | 0.84% residual activity at 20 mM | Aspergillus awamori | |
Cd2+ | 11.82% residual activity at 20 mM | Aspergillus awamori | |
Co2+ | 39.06% residual activity at 20 mM | Aspergillus awamori | |
Cu2+ | 10.58% residual activity at 20 mM | Aspergillus awamori | |
dithiothreitol | displays inhibitory properties at higher concentrations of 1.0% to 5.0% (v/v) | Aspergillus awamori | |
H2O2 | H2O2 concentrations of less than 2% and higher than 10% lead to a decline in enzyme activity | Aspergillus awamori | |
Hg2+ | 53.02% residual activity at 20 mM | Aspergillus awamori | |
Li+ | 3.07% residual activity at 20 mM | Aspergillus awamori | |
Mg2+ | 7.03% residual activity at 20 mM | Aspergillus awamori | |
Mn2+ | 10.18% residual activity at 20 mM | Aspergillus awamori | |
Mo2+ | 22.48% residual activity at 20 mM | Aspergillus awamori | |
Zn2+ | 30.54% residual activity at 20 mM | Aspergillus awamori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
methyl gallate | at pH 2.0, 30°C | Aspergillus awamori |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Aspergillus awamori | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | 144.44% activity at 10 mM | Aspergillus awamori | |
K+ | 176.62% activity at 10 mM | Aspergillus awamori | |
Na+ | 120.37% activity at 20 mM | Aspergillus awamori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37800 | - |
6 * 37800, SDS-PAGE | Aspergillus awamori |
230000 | - |
gel filtration | Aspergillus awamori |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | about 70% activity after 1 h at 10% (v/v) | Aspergillus awamori |
acetonitrile | about 85% activity after 1 h at 10% (v/v) | Aspergillus awamori |
benzene | about 45% activity after 1 h at 10% (v/v) | Aspergillus awamori |
Brij-35 | the enzyme is not stable in the presence of Brij-35, with less than 82% residual activity at 0.1-1% (v/v) | Aspergillus awamori |
Butanol | about 70% activity after 1 h at 10% (v/v) | Aspergillus awamori |
chloroform | about 60% activity after 1 h at 10% (v/v) | Aspergillus awamori |
DMSO | at a 60% concentration of DMSO, the enzyme retains a 61% activity after 24 h | Aspergillus awamori |
Ethanol | about 80% activity after 1 h at 10% (v/v) | Aspergillus awamori |
hexane | about 80% activity after 1 h at 10% (v/v) | Aspergillus awamori |
isopropanol | about 70% activity after 1 h at 10% (v/v) | Aspergillus awamori |
Methanol | about 80% activity after 1 h at 10% (v/v) | Aspergillus awamori |
Triton X-100 | at high concentrations of between 0.6% (v/v) (79.06% residual activity) to 1.0% (v/v) (54.26% residual activity), lead to a loss of enzyme stability, although the enzyme is stable at a 0.2% (v/v) concentration (112.2% residual activity) with an enhanced enzyme activity at a 0.4% concentration (171.36% residual activity) | Aspergillus awamori |
Tween 80 | Tween 80 leads to an enhanced enzyme activity at up to a 0.6% (v/v) concentration (157.89% residual activity), but displays inhibitory properties at a high concentration of 1.0% (v/v) (83.5% residual activity) | Aspergillus awamori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus awamori | C7F6Y1 | - |
- |
Aspergillus awamori BTMFW032 | C7F6Y1 | - |
- |
Oxidation Stability | Organism |
---|---|
increases in hydrogen peroxide concentrations result in enhanced enzyme activity in the range of 2% (161.1% activity) to 10% (220% activity) | Aspergillus awamori |
Purification (Comment) | Organism |
---|---|
ultrafiltration and Sephadex G-200 gel filtration | Aspergillus awamori |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
436.7 | - |
crude extract, pH 2.0, 30°C | Aspergillus awamori |
2762 | - |
after 6.32fold purification, pH 2.0, 30°C | Aspergillus awamori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl gallate + H2O | best substrate | Aspergillus awamori | gallate + methanol | - |
? | |
methyl gallate + H2O | best substrate | Aspergillus awamori BTMFW032 | gallate + methanol | - |
? | |
propyl gallate + H2O | - |
Aspergillus awamori | gallate + propanol | - |
? | |
propyl gallate + H2O | - |
Aspergillus awamori BTMFW032 | gallate + propanol | - |
? | |
tannic acid + H2O | lowest activity | Aspergillus awamori | gallic acid + D-glucose | - |
? | |
tannic acid + H2O | lowest activity | Aspergillus awamori BTMFW032 | gallic acid + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 37800, SDS-PAGE | Aspergillus awamori |
Synonyms | Comment | Organism |
---|---|---|
tannin acyl hydrolase | - |
Aspergillus awamori |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Aspergillus awamori |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 80 | the enzyme activity declines markedly along with increases in temperature above 60°C | Aspergillus awamori |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | the enzyme is stable only at 30°C for 24 h, retaining about 60% activity. The residual activity is 72% at 40°C for 2 h, 54% at 50°C for 6 h, 53% at 60°C for 4 h, and 72% at 70°C for 1 h | Aspergillus awamori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2 | - |
two pH optima, pH 2.0 and pH 8.0, are recorded | Aspergillus awamori |
8 | - |
two pH optima, pH 2.0 and pH 8.0, are recorded | Aspergillus awamori |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
1 | 7 | the enzyme records a significant level of activity even at a pH of 1.0, however, the enzyme activity declines rapidly at pH 3.0-4.0 and is almost nil at a pH of 7.0 | Aspergillus awamori |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | - |
the enzyme is stable (99%) over a relatively long duration of incubation (up to 24 h) only at pH 2.0. At other pH levels, the enzyme is not stable | Aspergillus awamori |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aspergillus awamori | isoelectric focusing | - |
4.4 |