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Literature summary for 3.1.1.20 extracted from

  • Chhokar, V.; Seem, S.; Beniwal, V.; Salar, R.; Nehra, K.; Kumar, A.; Rana, J.
    Purification and characterization of extracellular tannin acyl hydrolase from Aspergillus heteromorphus MTCC 8818 (2010), Biotechnol. Bioprocess Eng., 15, 793-799.
No PubMed abstract available
Search for more literature for 3.1.1.20

Activating Compound

Activating Compound Comment Organism Structure
sodium lauryl sulfate 45% increase of activity at 1% (v/v) Aspergillus heteromorphus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 42% inhibition at 1 mM Aspergillus heteromorphus
4-Aminobenzoic acid 81.9% residual activity at 1 mM Aspergillus heteromorphus
Ag+ 57% residual activity at 5 mM Aspergillus heteromorphus
Benzene 98% residual activity at 5% (v/v) Aspergillus heteromorphus
benzoic acid 83.1% residual activity at 1 mM Aspergillus heteromorphus
beta-mercaptoethanol 59.4% residual activity at 1 mM Aspergillus heteromorphus
Cd2+ 57% residual activity at 5 mM Aspergillus heteromorphus
Co2+ 87.6% residual activity at 1 mM Aspergillus heteromorphus
EDTA 35% inhibition at 1 mM Aspergillus heteromorphus
ethanol 80.2% residual activity at 5% (v/v) Aspergillus heteromorphus
gallic acid 82.1% residual activity at 1 mM Aspergillus heteromorphus
Hg2+ 56% residual activity at 5 mM Aspergillus heteromorphus
Isopropanol 96.3% residual activity at 5% (v/v) Aspergillus heteromorphus
K+ 73.3% residual activity at 5 mM Aspergillus heteromorphus
methanol 95.8% residual activity at 5% (v/v) Aspergillus heteromorphus
Mg2+ 77.5% residual activity at 5 mM Aspergillus heteromorphus
Mn2+ 79% residual activity at 1 mM Aspergillus heteromorphus
additional information not inhibited by iodoacetamide Aspergillus heteromorphus
N-bromosuccinimide 73.9% residual activity at 1 mM Aspergillus heteromorphus
Na+ 89.1% residual activity at 5 mM Aspergillus heteromorphus
phenylmethylsulfonyl fluoride 86.1% residual activity at 1 mM Aspergillus heteromorphus
propyl gallate 85.1% residual activity at 1 mM Aspergillus heteromorphus
pyrogallol 95% residual activity at 1 mM Aspergillus heteromorphus
Sodium azide 82.8% residual activity at 1 mM Aspergillus heteromorphus
Sodium bisulfite 81.3% residual activity at 1 mM Aspergillus heteromorphus
Sodium thioglycolate 83.1% residual activity at 1 mM Aspergillus heteromorphus
Triton X-100 21.9% inhibition at 1% (v/v) Aspergillus heteromorphus
Tween 20 30.8% inhibition at 1% (v/v) Aspergillus heteromorphus
Tween 80 27.4% inhibition at 1% (v/v) Aspergillus heteromorphus
Zn2+ 80% residual activity at 5 mM Aspergillus heteromorphus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Aspergillus heteromorphus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 105.6% activity at 1 mM Aspergillus heteromorphus
Cu+ 108.6% activity at 1 mM Aspergillus heteromorphus
Cu2+ 106.2% activity at 1 mM Aspergillus heteromorphus
Fe2+ 107.6% activity at 1 mM Aspergillus heteromorphus
additional information Ba2+ has no significant effect on tannase activity up to 3 mM Aspergillus heteromorphus

Organic Solvent Stability

Organic Solvent Comment Organism
benzene 116.7% activity at 1% (v/v) Aspergillus heteromorphus
Butanol 115.5% residual activity at 5% (v/v) Aspergillus heteromorphus
DMSO 122.4% residual activity at 5% (v/v) Aspergillus heteromorphus
Ethanol 104.4% residual activity at 1% (v/v) Aspergillus heteromorphus
Glycerol 118.2% residual activity at 1% (v/v) Aspergillus heteromorphus
isopropanol 106.6% activity at 1% (v/v) Aspergillus heteromorphus
Methanol 103.2% residual activity at 1% (v/v) Aspergillus heteromorphus
toluene 119.4% residual activity at 1% (v/v) Aspergillus heteromorphus

Organism

Organism UniProt Comment Textmining
Aspergillus heteromorphus
-
-
-
Aspergillus heteromorphus MTCC 8818
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and DEAE-cellulose column chromatography (overall purification of 39.74fold with a yield of 19.29%) Aspergillus heteromorphus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.575
-
 crude extract, pH 5.5, 50°C Aspergillus heteromorphus
22.85
-
 after 39.74fold purification, pH 5.5, 50°C Aspergillus heteromorphus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tannic acid + H2O
-
 Aspergillus heteromorphus gallic acid + D-glucose
-
 ?
tannic acid + H2O
-
 Aspergillus heteromorphus MTCC 8818 gallic acid + D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
tannin acyl hydrolase
-
 Aspergillus heteromorphus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Aspergillus heteromorphus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Aspergillus heteromorphus