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Literature summary for 3.1.1.20 extracted from

  • Mata-Gomez, M.; Rodriguez, L.V.; Ramos, E.L.; Renovato, J.; Cruz-Hernandez, M.A.; Rodriguez, R.; Contreras, J.; Aguilar, C.N.
    A novel tannase from the xerophilic fungus Aspergillus niger GH1 (2009), J. Microbiol. Biotechnol., 19, 987-996.
    View publication on PubMed
Search for more literature for 3.1.1.20

Activating Compound

Activating Compound Comment Organism Structure
heptane at 20% of concentration increases activity by 19% after 60 min, and at 60% of concentration by 97% after 6 min Aspergillus niger
additional information Tween 20 and Triton X-100 have no effect Aspergillus niger
petroleum ether at 20% of concentration increases activity by 19% after 60 min, and at 60% of concentration by 73% after 6 min Aspergillus niger
Tween 80 at 0.1% of concentration increases activity by 25% after 60 min Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
acetone at 60% of concentration, completely inhibits activity at 30°C Aspergillus niger
Cu2+ ca. 20% inhibition after 60 min of incubation at 30°C and pH 5 Aspergillus niger
EDTA at 0.01% of concentration 22% inhibition after 5 min and at 0.1% of concentration 19% inhibition after 60 min at 30°C Aspergillus niger
ethanol at 60% of concentration, completely inhibits activity at 30°C Aspergillus niger
Fe3+ more than 40% inhibition after 5 min and 60 min of incubation at 30°C and pH 5 Aspergillus niger
formaldehyde 51.5% inhibition at 20% after 60 min at 30°C Aspergillus niger
heptane at 60% of concentration 95% inhibition after 60 min at 30°C Aspergillus niger
Mg2+ ca. 20% inhibition after 5 min of incubation at 30°C and pH 5 Aspergillus niger
Mn2+ ca. 20% inhibition after 5 min of incubation at 30°C and pH 5 Aspergillus niger
additional information at 0.1% and 0.01% of concentration, Ca2+, Tween 20 and Triton X-100 have no effect at 30°C Aspergillus niger
phenylmethylsulfonyl fluoride 38% inhibition after 5 min of incubation at 30°C and pH 5, indicating the presence of a serine or cysteine residue in the catalytic site Aspergillus niger
SDS at 0.01% of concentration 15% inhibition after 60 min at 30°C Aspergillus niger
tetrahydrofuran at 60% of concentration, completely inhibits activity at 30°C Aspergillus niger
Tween 80 at 0.01% of concentration 35% inhibition after 5 min at 30°C Aspergillus niger
Zn2+ ca. 10% inhibition after 5 min and 60 min of incubation at 30°C and pH 5 Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
 Tannic acid at 60°C Aspergillus niger
0.41
-
 Methyl 3,4,5-trihydroxybenzoate at 60°C Aspergillus niger

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
 Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 1 * 50000 + 1 * 75000 + 1 * 100000, SDS-PAGE Aspergillus niger
75000
-
 1 * 50000 + 1 * 75000 + 1 * 100000, SDS-PAGE Aspergillus niger
100000
-
 1 * 50000 + 1 * 75000 + 1 * 100000, SDS-PAGE Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger GH1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to apparent homogeneity, by ultrafiltration, anion-exchange chromatography and gel filtration, with a final yield of 0.3%, 46fold Aspergillus niger

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 the crude extract initially has a specific activity of 5.2 IU/mg. The 46fold purified enzyme has a specific activity of 238.14 IU/mg Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl 3,4,5-trihydroxybenzoate + H2O
-
 Aspergillus niger methanol + 3,4,5-trihydroxybenzoate
-
 ?
methyl 3,4,5-trihydroxybenzoate + H2O
-
 Aspergillus niger GH1 methanol + 3,4,5-trihydroxybenzoate
-
 ?
tannic acid + H2O
-
 Aspergillus niger gallic acid + D-glucose
-
 ?
tannic acid + H2O
-
 Aspergillus niger GH1 gallic acid + D-glucose
-
 ?

Subunits

Subunits Comment Organism
trimer 1 * 50000 + 1 * 75000 + 1 * 100000, SDS-PAGE Aspergillus niger

Synonyms

Synonyms Comment Organism
tannase
-
 Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 with 0.1 M citrate buffer, pH 5.5 Aspergillus niger

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 at 20°C exhibits a relative activity of 10% Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 70 half-lives of the enzyme at 50, 60, and 70°C are 281, 25, and 4 min, respectively Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 at 30°C, using methyl 3,4,5-trihydroxybenzoate as substrate Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
2 6 at pH 2.0 exhibits a relative activity of about 45% Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 6 in the pH range 3.0-5.0, tannase proves to be significantly stable, showing half-lives ranging from 131 to 372 h. At pH 6.0 and 5.5 half-lives of 632 h and 607 h, respectively Aspergillus niger