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Literature summary for 3.1.1.20 extracted from

  • Curiel, J.A.; Rodriguez, H.; Acebron, I.; Mancheno, J.M.; De Las Rivas, B.; Munoz, R.
    Production and physicochemical properties of recombinant Lactobacillus plantarum tannase (2009), J. Agric. Food Chem., 57, 6224-6230.
    View publication on PubMed
Search for more literature for 3.1.1.20

Application

Application Comment Organism
food industry the use of Lactobacillus plantarum tannase is an adequate alternative to the fungal tannases currently used in the food industry. Use of tannase may provide an efficient means for obtaining molecules with valuable activities from the degradation of complex tannins present in food and agricultural wastes Lactiplantibacillus plantarum

Cloned(Commentary)

Cloned (Comment) Organism
1.4 kb purified PCR product of tanLp1 gene cloned into pURI3 vector and transformed into Escherichia coli DH5alpha cells. Recombinant plasmids isolated and overexpressed in Escherichia coli JM109 (DE3) Lactiplantibacillus plantarum

Inhibitors

Inhibitors Comment Organism Structure
beta-mercaptoethanol 1 mM inhibits activity by 85% Lactiplantibacillus plantarum
ethyl gallate 1 mM inhibits activity by 48% Lactiplantibacillus plantarum
Hg2+ 1 mM inhibits activity by 78% Lactiplantibacillus plantarum
Mg2+ 1 mM inhibits activity by 12% Lactiplantibacillus plantarum
additional information 1 mM DMSO has no significant effect Lactiplantibacillus plantarum
Triton X 100 1 mM inhibits activity by 20% Lactiplantibacillus plantarum
Tween 1 mM inhibits activity by 20% Lactiplantibacillus plantarum
Urea 1 mM inhibits activity by 52% Lactiplantibacillus plantarum
Zn2+ 1 mM inhibits activity by 18% Lactiplantibacillus plantarum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM increases activity by 81% Lactiplantibacillus plantarum
K+ 1 mM increases activity by 26% Lactiplantibacillus plantarum
additional information 1 mM EDTA has no significant effect Lactiplantibacillus plantarum

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum
-
-
-
Lactiplantibacillus plantarum CECT 748 / ATCC 14917 / DSM 20174
-
-
-

Purification (Commentary)

Purification (Comment) Organism
on affinity column Lactiplantibacillus plantarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
epigallocatechin gallate + H2O
-
 Lactiplantibacillus plantarum gallic acid + epigallocatechin
-
 ?
epigallocatechin gallate + H2O
-
 Lactiplantibacillus plantarum CECT 748 / ATCC 14917 / DSM 20174 gallic acid + epigallocatechin
-
 ?
ethyl gallate + H2O
-
 Lactiplantibacillus plantarum gallic acid + ethanol
-
 ?
ethyl gallate + H2O
-
 Lactiplantibacillus plantarum CECT 748 / ATCC 14917 / DSM 20174 gallic acid + ethanol
-
 ?
gallocatechin gallate + H2O
-
 Lactiplantibacillus plantarum gallic acid + gallocatechin
-
 ?
gallocatechin gallate + H2O
-
 Lactiplantibacillus plantarum CECT 748 / ATCC 14917 / DSM 20174 gallic acid + gallocatechin
-
 ?
lauryl gallate + H2O
-
 Lactiplantibacillus plantarum gallic acid + lauric acid
-
 ?
methyl gallate + H2O recombinant tannase shows similar affinity for both methyl gallate and ethyl gallate Lactiplantibacillus plantarum gallate + methanol
-
 ?
methyl gallate + H2O recombinant tannase shows similar affinity for both methyl gallate and ethyl gallate Lactiplantibacillus plantarum CECT 748 / ATCC 14917 / DSM 20174 gallate + methanol
-
 ?
additional information tannases hydrolyze only those substrates that contain at least two phenolic OH groups in the acid component. The esterified COOH group must be on the oxidized benzene ring and must not be ortho to one of the OH groups. Benzoic esters (methyl benzoate, and ethyl benzoate), hydroxybenzoic esters (methyl 4-hydroxybenzoate, ethyl 4-hydroxybenzoate, propyl 4-hydroxybenzoate, and butyl 4-hydroxybenzoate), vanillic ester (methyl vanillate), gentisic ester (methyl gentisate), salicylic ester (methyl salicylate), ferulic esters (ferulic methyl ester and ferulic ethyl ester), ellagic, chlorogenic acids, quercetin, catechin, epicatechin, gallocatechin, epigallocatechin or 4-nitrophenyl beta-D-glucopyranoside are not metabolized Lactiplantibacillus plantarum ?
-
 ?
additional information tannases hydrolyze only those substrates that contain at least two phenolic OH groups in the acid component. The esterified COOH group must be on the oxidized benzene ring and must not be ortho to one of the OH groups. Benzoic esters (methyl benzoate, and ethyl benzoate), hydroxybenzoic esters (methyl 4-hydroxybenzoate, ethyl 4-hydroxybenzoate, propyl 4-hydroxybenzoate, and butyl 4-hydroxybenzoate), vanillic ester (methyl vanillate), gentisic ester (methyl gentisate), salicylic ester (methyl salicylate), ferulic esters (ferulic methyl ester and ferulic ethyl ester), ellagic, chlorogenic acids, quercetin, catechin, epicatechin, gallocatechin, epigallocatechin or 4-nitrophenyl beta-D-glucopyranoside are not metabolized Lactiplantibacillus plantarum CECT 748 / ATCC 14917 / DSM 20174 ?
-
 ?
propyl gallate + H2O
-
 Lactiplantibacillus plantarum gallic acid + propanol
-
 ?
protocatechuic acid ethyl ester + H2O
-
 Lactiplantibacillus plantarum protocatechuate + ethanol
-
 ?
tannic acid + H2O
-
 Lactiplantibacillus plantarum gallic acid + D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
tannase
-
 Lactiplantibacillus plantarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 purified recombinant tannase Lactiplantibacillus plantarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 purified recombinant tannase Lactiplantibacillus plantarum

pH Range

pH Minimum pH Maximum Comment Organism
6 8
-
 Lactiplantibacillus plantarum