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Literature summary for 3.1.1.20 extracted from
- Purification, immobilization and characterization of tannase from Penicillium variable (2008), Bioresour. Technol., 99, 2544-2551.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sodium cholate | - |
Talaromyces variabilis |  |
| sodium taurocholate | - |
Talaromyces variabilis | |
General Stability
| General Stability | Organism |
|---|---|
| the tannase can successfully be immobilized on Amberlite IR where it retains about 85% of the initial catalytic activity even after the ninth cycle of its use | Talaromyces variabilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 40% residual activity at 1 mM | Talaromyces variabilis | |
| 1-[(naphthalen-1-yl)oxy]-3-[(propan-2-yl)amino]propan-2-ol | 49.6% residual activity at 1 mM | Talaromyces variabilis | |
| 8-hydroxyquinoline | 47.2% residual activity at 1 mM | Talaromyces variabilis | |
| Bromoacetic acid | 53.3% residual activity at 1 mM | Talaromyces variabilis | |
| cyanamide | 61.7% residual activity at 1 mM | Talaromyces variabilis | |
| dithiothreitol | 54.1% residual activity at 1 mM | Talaromyces variabilis | |
| iodoacetic acid | 48% residual activity at 1 mM | Talaromyces variabilis | |
| mercuribenzoic acid | 48.8% residual activity at 1 mM | Talaromyces variabilis | |
| N-ethylmaleimide | 7% residual activity at 1 mM | Talaromyces variabilis | |
| phenyl boronic acid | 67.1% residual activity at 1 mM | Talaromyces variabilis | |
| phenylmethylsulfonyl fluoride | 28% residual activity at 1 mM | Talaromyces variabilis | |
| sodium cholate | 52.6% residual activity at 1 mM | Talaromyces variabilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12 | - |
propyl gallate | in 0.5 M citrate phosphate buffer, pH 5.0, at 50°C | Talaromyces variabilis | |
| 14 | - |
methyl gallate | in 0.5 M citrate phosphate buffer, pH 5.0, at 50°C | Talaromyces variabilis | |
| 32 | - |
Tannic acid | in 0.5 M citrate phosphate buffer, pH 5.0, at 50°C | Talaromyces variabilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 155000 | - |
2 * 155000, gel filtration | Talaromyces variabilis |
| 158000 | - |
2 * 158000, SDS-PAGE | Talaromyces variabilis |
| 310000 | - |
gel filtration | Talaromyces variabilis |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| 2-mercaptomethanol | 39.6% residual activity at 1 mM | Talaromyces variabilis |
| Acetone | 49% residual activity after 60 min at 20% (v/v) | Talaromyces variabilis |
| carbon tetrachloride | 57% residual activity after 60 min at 60% (v/v) | Talaromyces variabilis |
| Ethanol | 35.7% residual activity after 60 min at 60% (v/v) | Talaromyces variabilis |
| formaldehyde | 12% residual activity after 5 min at 20% (v/v) | Talaromyces variabilis |
| heptane | 62% residual activity after 60 min at 60% (v/v) | Talaromyces variabilis |
| additional information | not affected by Triton X-100 | Talaromyces variabilis |
| petroleum ether | 33% residual activity after 60 min at 60% (v/v) | Talaromyces variabilis |
| SDS | complete inactivation at 1% (w/v) | Talaromyces variabilis |
| tetrahydrofuran | no activity after 5 min at 20% (v/v) | Talaromyces variabilis |
| toluene | 44% residual activity after 60 min at 60% (v/v) | Talaromyces variabilis |
| Tween 60 | complete inactivation at 1% (v/v) | Talaromyces variabilis |
| Tween 80 | complete inactivation at 1% (v/v) | Talaromyces variabilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Talaromyces variabilis | - |
- |
- |
| Talaromyces variabilis IARI 2031 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ultrafiltration and Sephadex G-200 gel filtration | Talaromyces variabilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15.27 | - |
crude filtrate, in 0.5 M citrate phosphate buffer, pH 5.0, at 50°C | Talaromyces variabilis |
| 2055 | - |
after 135fold purification, in 0.5 M citrate phosphate buffer, pH 5.0, at 50°C | Talaromyces variabilis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C and 30°C, tannase in both crude and crude lyophilized forms, one year, retains more than 60% residual activity | Talaromyces variabilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methyl gallate + H2O | - |
Talaromyces variabilis | gallic acid + methanol | - |
? | |
| methyl gallate + H2O | - |
Talaromyces variabilis IARI 2031 | gallic acid + methanol | - |
? | |
| n-propyl gallate + H2O | - |
Talaromyces variabilis | gallic acid + n-propanol | - |
? | |
| n-propyl gallate + H2O | - |
Talaromyces variabilis IARI 2031 | gallic acid + n-propanol | - |
? | |
| tannic acid + H2O | best substrate | Talaromyces variabilis | gallate + D-glucose | - |
? | |
| tannic acid + H2O | best substrate | Talaromyces variabilis IARI 2031 | gallate + D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 158000, SDS-PAGE | Talaromyces variabilis |
| homodimer | 2 * 155000, gel filtration | Talaromyces variabilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tannin acyl hydrolase | belongs to the class of serine hydrolases | Talaromyces variabilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Talaromyces variabilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 80 | - |
Talaromyces variabilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 90 | at 25°C crude and immobilized enzyme show 78% and 81% relative activity while purified tannase has a relative activity of 70% at this temperature, at 80°C more than 50% relative activity is observed in crude and immobilized tannase while 45% in purified form, at 90°C crude and immobilized enzyme retain 23% and 31% relative activity while purified tannase shows a relative activity of only 11% | Talaromyces variabilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Talaromyces variabilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | - |
Talaromyces variabilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 10 | the enzyme shows 60% residual activity at pH 3.0, almost 100% stability in pH range of 4.0-6.0 for 24 h, at pH 10.0 crude and purified tannase retain 19.7% and 15.3% residual activity for 24 h, immobilized tannase retains 24.6% residual activity at this pH even after 24 h | Talaromyces variabilis |
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