Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
4-chloromercuribenzoate | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
Ag+ | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
alpha-glutathione | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
Cd2+ | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
Co2+ | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
CO32- | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
Cu2+ | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
Fe3+ | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
Hg2+ | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
K+ | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
Mn2+ | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
Pb2+ | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
Sn2+ | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
sodium dodecyl sulphate | inhibits isozyme TAH II | Hypocreales sp. | |
Triton X-100 | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
Tween 20 | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
Tween 60 | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
Tween 80 | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
Zn2+ | inhibits both isozymes TAH I and TAH II | Hypocreales sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.05 | - |
Tannic acid | isozyme TAH I, pH 5.5, 20°C | Hypocreales sp. | |
1.51 | - |
Tannic acid | isozyme TAH II, pH 5.5, 20°C | Hypocreales sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Hypocreales sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Br- | activates both isozymes TAH I and TAH II | Hypocreales sp. | |
Mg2+ | activates both isozymes TAH I and TAH II | Hypocreales sp. | |
Urea | activates both isozymes TAH I and TAH II | Hypocreales sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, isozyme TAH I, SDS-PAGE, x * 46000, isozyme TAH II, SDS-PAGE | Hypocreales sp. |
46000 | - |
x * 40000, isozyme TAH I, SDS-PAGE, x * 46000, isozyme TAH II, SDS-PAGE | Hypocreales sp. |
155000 | - |
isozymes TAH I and TAH II, gel filtration | Hypocreales sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hypocreales sp. | - |
Antarctic strain P9, two cold-adapted isozymes TAH I and TAH II | - |
Purification (Comment) | Organism |
---|---|
native isozymes TAH I and TAH II 7.9fold and 10.5fold, respectively, to homogeneity | Hypocreales sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
61.8 | - |
purified isozyme TAH I | Hypocreales sp. |
82.2 | - |
purified isozyme TAH II | Hypocreales sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+/-)-epicatechin-gallate + H2O | - |
Hypocreales sp. | epicatechin + gallate | - |
? | |
(+/-)-epigallocatechin-3-gallate + H2O | - |
Hypocreales sp. | epigallocatechin + gallate | - |
? | |
m-digallate + H2O | - |
Hypocreales sp. | gallate | - |
? | |
additional information | tannase cleaves ester bonds between gallic acid and glucose in tannic acid and m-digallic acid ester linkages, named depside bonds, it also hydrolyzes (+)-epicatechin gallate and (+)-epigallocatechin-3-gallate | Hypocreales sp. | ? | - |
? | |
tannic acid + H2O | - |
Hypocreales sp. | gallate + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, isozyme TAH I, SDS-PAGE, x * 46000, isozyme TAH II, SDS-PAGE | Hypocreales sp. |
Synonyms | Comment | Organism |
---|---|---|
TAH | - |
Hypocreales sp. |
tannin acyl hydrolase | - |
Hypocreales sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
isozyme TAH II | Hypocreales sp. |
25 | - |
isozyme TAH I | Hypocreales sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
temperature profile of the isozymes | Hypocreales sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
temperature profile of the isozymes | Hypocreales sp. |
20 | 30 | isozymes TAH I and TAH II, stable | Hypocreales sp. |
40 | 50 | isozymes TAH I and TAH II, loss of 50-80% activity | Hypocreales sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
isozymes TAH I and TAH II | Hypocreales sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7.5 | loss of 10-30% activity of TAH I and TAH II at "5°C and 4°C | Hypocreales sp. |