Literature summary for 3.1.1.11 extracted from

Dorokhov, Y.L.; Frolova, O.Y.; Skurat, E.V.; Ivanov, P.A.; Gasanova, T.V.; Sheveleva, A.A.; Ravin, N.V.; Maekinen, K.M.; Klimyuk, V.I.; Skryabin, K.G.; Gleba, Y.Y.; Atabekov, J.G.
A novel function for a ubiquitous plant enzyme pectin methylesterase: the enhancer of RNA silencing (2006), FEBS Lett., 580, 3872-3878.

Search for more literature for 3.1.1.11

Cloned(Commentary)

Cloned (Comment)	Organism
transient co-expression of the tobacco enzyme with Tobacco mosaic virus TMV-GFP fusion protein in Nicotiana benthamiana plants via transfection mediated by Agrobacterium tumefaciens strain GV3110, the co-expression results in increased virus-induced RNA silencing with inhibition of GFP production, virus RNA degradation, stimulation of siRNAs production, overview	Nicotiana benthamiana

Protein Variants

Protein Variants	Comment	Organism
395A396A	an enzymatically inactive proPME mutant	Nicotiana benthamiana
additional information	expression of proPME enhances the GFP transgene-induced gene silencing accompanied by relocation of the DCL1 protein from nucleus to the cytoplasm and activation of siRNAs and miRNAs production, inhibition of proPME gene expression stimulates the TMV vector reproduction, overview	Nicotiana benthamiana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
pectin + H2O	Nicotiana benthamiana	-	methanol + pectate	-	?

Organism

Organism	UniProt	Comment	Textmining
Nicotiana benthamiana	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	the gene encoding PME has an N-terminal extension encoding the pro-sequence	Nicotiana benthamiana

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Nicotiana benthamiana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pectin + H2O	-	Nicotiana benthamiana	methanol + pectate	-	?

Synonyms

Synonyms	Comment	Organism
pectin methylesterase	-	Nicotiana benthamiana
PME	-	Nicotiana benthamiana

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Release 2023.1 (January 2023)