Application | Comment | Organism |
---|---|---|
diagnostics | pectin methylesterase is an allergenic marker for the sensitization to Russian thistle, Salsola kali pollen | Kali turgidum |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression in Escherichia coli | Kali turgidum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37214 | - |
x * 40000, native enzyme, SDS-PAGE, x * 37214, native glycosylated enzyme, mass spectrometry | Kali turgidum |
40000 | - |
x * 40000, native enzyme, SDS-PAGE, x * 37214, native glycosylated enzyme, mass spectrometry | Kali turgidum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Kali turgidum | the mildly basic and polymorphic protein causes allergic reactions in humans determined by secific IgE production | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kali turgidum | Q17ST3 | frgament | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | carbohydrate detection, overview | Kali turgidum |
Purification (Comment) | Organism |
---|---|
native enzyme from pollen by gel filtration, anion exchange chromatography and isoelectric focusing | Kali turgidum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
pectin + n H2O = n methanol + pectate | the catalytic residues Gln151, Gln173, Asp174, Asp195, and Arg253 are conserved in Sal k 1 | Kali turgidum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pollen | - |
Kali turgidum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the mildly basic and polymorphic protein causes allergic reactions in humans determined by secific IgE production | Kali turgidum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, native enzyme, SDS-PAGE, x * 37214, native glycosylated enzyme, mass spectrometry | Kali turgidum |
More | model of the three-dimensional structure with conserved parallel beta-sheet coiled into a large, right-handed cylinder based on the structure of the Erwinia chrysantemi enzyme with PDB ID 1QJV, overview | Kali turgidum |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the plant pectin methylesterase family | Kali turgidum |
pectine methylesterase | - |
Kali turgidum |
PME | - |
Kali turgidum |
Sal k 1 | - |
Kali turgidum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Kali turgidum | due to heterogenities | 7.8 | 6.7 |