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Literature summary for 3.1.1.11 extracted from

  • Markovic, O.; Machova, E.
    Immobilization of pectin esterase from tomatoes and Aspergillus foetidus on various supports (1985), Collect. Czech. Chem. Commun., 50, 2021-2027.
No PubMed abstract available

General Stability

General Stability Organism
increased temperature stability after immobilization Solanum lycopersicum
increased temperature stability after immobilization Aspergillus foetidus

Organism

Organism UniProt Comment Textmining
Aspergillus foetidus
-
-
-
Solanum lycopersicum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information immobilized enzymes show about 7.5% of the activity of the free enzyme Solanum lycopersicum ?
-
?
additional information enzyme immobilized on CNBr-Sepharose 4B show about 11.5% of the activity of the free enzyme Aspergillus foetidus ?
-
?
additional information enzyme immobilized on polyethylene terephthalate shows 23.1% of the activity of the free enzyme Aspergillus foetidus ?
-
?
additional information the immobilized enzyme, unlike the free pectin esterase, does not act on pectin showing a higher esterification degree Solanum lycopersicum ?
-
?
additional information the immobilized enzyme, unlike the free pectin esterase, does not act on pectin showing a higher esterification degree Aspergillus foetidus ?
-
?
pectin + H2O
-
Solanum lycopersicum methanol + pectate
-
?
pectin + H2O
-
Aspergillus foetidus methanol + pectate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
soluble enzyme Aspergillus foetidus
50
-
soluble enzyme Solanum lycopersicum
50
-
CNBr-Sepharose 4B immobilized enzyme Aspergillus foetidus
52
-
enzyme immobilized on polyethylene terephthalate Aspergillus foetidus
61
-
enzyme immobilized on polyethylene terephthalate Solanum lycopersicum
62
-
enzyme immobilized on pectin esterase Solanum lycopersicum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
20 min, about 50% loss of activity of the soluble enzyme, about 20% loss of activity of the immobilized enzyme Solanum lycopersicum
65
-
20 min, complete loss of activity of the soluble enzyme, about 50% loss of activity of the immobilized enzyme Solanum lycopersicum
65
-
20 min, about 75% loss of activity of the soluble enzyme, about 20% loss of activity of the immobilized enzyme Aspergillus foetidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6
-
enzyme immobilized on CNBr-Sepharose 4B or polyethylenen terephthalate Aspergillus foetidus
4.8
-
soluble enzyme Aspergillus foetidus
7.5
-
soluble and immobilized enzyme Solanum lycopersicum