General Stability | Organism |
---|---|
increased temperature stability after immobilization | Solanum lycopersicum |
increased temperature stability after immobilization | Aspergillus foetidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus foetidus | - |
- |
- |
Solanum lycopersicum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | immobilized enzymes show about 7.5% of the activity of the free enzyme | Solanum lycopersicum | ? | - |
? | |
additional information | enzyme immobilized on CNBr-Sepharose 4B show about 11.5% of the activity of the free enzyme | Aspergillus foetidus | ? | - |
? | |
additional information | enzyme immobilized on polyethylene terephthalate shows 23.1% of the activity of the free enzyme | Aspergillus foetidus | ? | - |
? | |
additional information | the immobilized enzyme, unlike the free pectin esterase, does not act on pectin showing a higher esterification degree | Solanum lycopersicum | ? | - |
? | |
additional information | the immobilized enzyme, unlike the free pectin esterase, does not act on pectin showing a higher esterification degree | Aspergillus foetidus | ? | - |
? | |
pectin + H2O | - |
Solanum lycopersicum | methanol + pectate | - |
? | |
pectin + H2O | - |
Aspergillus foetidus | methanol + pectate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
soluble enzyme | Aspergillus foetidus |
50 | - |
soluble enzyme | Solanum lycopersicum |
50 | - |
CNBr-Sepharose 4B immobilized enzyme | Aspergillus foetidus |
52 | - |
enzyme immobilized on polyethylene terephthalate | Aspergillus foetidus |
61 | - |
enzyme immobilized on polyethylene terephthalate | Solanum lycopersicum |
62 | - |
enzyme immobilized on pectin esterase | Solanum lycopersicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
20 min, about 50% loss of activity of the soluble enzyme, about 20% loss of activity of the immobilized enzyme | Solanum lycopersicum |
65 | - |
20 min, complete loss of activity of the soluble enzyme, about 50% loss of activity of the immobilized enzyme | Solanum lycopersicum |
65 | - |
20 min, about 75% loss of activity of the soluble enzyme, about 20% loss of activity of the immobilized enzyme | Aspergillus foetidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.6 | - |
enzyme immobilized on CNBr-Sepharose 4B or polyethylenen terephthalate | Aspergillus foetidus |
4.8 | - |
soluble enzyme | Aspergillus foetidus |
7.5 | - |
soluble and immobilized enzyme | Solanum lycopersicum |