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Literature summary for 3.1.1.1 extracted from

  • Li, X.J.; Zheng, R.C.; Wu, Z.M.; Ding, X.; Zheng, Y.G.
    Thermophilic esterase from Thermomyces lanuginosus molecular cloning, functional expression and biochemical characterization (2014), Protein Expr. Purif., 101, 1-7 .
    View publication on PubMed
Search for more literature for 3.1.1.1

Application

Application Comment Organism
synthesis enzyme shows high enantioselectivity in the kinetic resolution of 2-carboxyethyl-3-cyano-5-methylhexanoic acid ethyl ester, which produce a valuable chiral intermediate-(3S)-2-carboxyethyl-3-cyano-5-methylhexanoic acid for the drug Pregabalin Thermomyces lanuginosus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermomyces lanuginosus

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+
-
 Thermomyces lanuginosus
Ca2+
-
 Thermomyces lanuginosus

Organism

Organism UniProt Comment Textmining
Thermomyces lanuginosus W8DVW0
-
-
Thermomyces lanuginosus DSM 10635 W8DVW0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-carboxyethyl-3-cyano-5-methylhexanoic acid ethyl ester + H2O
-
 Thermomyces lanuginosus (3S)-2-carboxyethyl-3-cyano-5-methylhexanoic acid + ethanol
-
 ?
4-nitrophenyl acetate + H2O about 55% of the activity with 4-nitrophenyl butanoate Thermomyces lanuginosus 4-nitrophenol + acetate
-
 ?
4-nitrophenyl acetate + H2O about 55% of the activity with 4-nitrophenyl butanoate Thermomyces lanuginosus DSM 10635 4-nitrophenol + acetate
-
 ?
4-nitrophenyl butanoate + H2O best substrate Thermomyces lanuginosus 4-nitrophenol + butanoate
-
 ?
4-nitrophenyl decanoate + H2O about 10% of the activity with 4-nitrophenyl butanoate Thermomyces lanuginosus 4-nitrophenol + decanoate
-
 ?
4-nitrophenyl decanoate + H2O about 10% of the activity with 4-nitrophenyl butanoate Thermomyces lanuginosus DSM 10635 4-nitrophenol + decanoate
-
 ?
4-nitrophenyl hexanoate + H2O about 65% of the activity with 4-nitrophenyl butanoate Thermomyces lanuginosus 4-nitrophenol + hexanoate
-
 ?
4-nitrophenyl hexanoate + H2O about 65% of the activity with 4-nitrophenyl butanoate Thermomyces lanuginosus DSM 10635 4-nitrophenol + hexanoate
-
 ?
4-nitrophenyl octanoate + H2O about 30% of the activity with 4-nitrophenyl butanoate Thermomyces lanuginosus 4-nitrophenol + octanoate
-
 ?
4-nitrophenyl octanoate + H2O about 30% of the activity with 4-nitrophenyl butanoate Thermomyces lanuginosus DSM 10635 4-nitrophenol + octanoate
-
 ?
additional information no substrate: 4-nitrophenyl palmitate Thermomyces lanuginosus ?
-
 ?
additional information no substrate: 4-nitrophenyl palmitate Thermomyces lanuginosus DSM 10635 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 34500, calculated, x * 35000, SDS-PAGE Thermomyces lanuginosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Thermomyces lanuginosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
65
-
 80% of maximum activtiy Thermomyces lanuginosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 8 h, 50% resiudal activity Thermomyces lanuginosus
70
-
 60 min, 53% residual activity Thermomyces lanuginosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Thermomyces lanuginosus

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9 low activity below or above Thermomyces lanuginosus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 12 h, 80% residual activity Thermomyces lanuginosus