Literature summary for 3.1.1.1 extracted from

Hiblot, J.; Bzdrenga, J.; Champion, C.; Chabriere, E.; Elias, M.
Crystal structure of VmoLac, a tentative quorum quenching lactonase from the extremophilic crenarchaeon Vulcanisaeta moutnovskia (2015), Sci. Rep., 5, 8372.

Search for more literature for 3.1.1.1

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method at 20°C, X-ray structures of the enzyme bound to a fatty acid and to its substrate acyl-homoserine lactone	Vulcanisaeta moutnovskia

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4.47	-	4-nitrophenyl acetate	pH 6.5, 50°C	Vulcanisaeta moutnovskia

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	the enzyme possesses a bi-cobalt active site	Vulcanisaeta moutnovskia

Organism

Organism	UniProt	Comment	Textmining
Vulcanisaeta moutnovskia	F0QXN6	-	-
Vulcanisaeta moutnovskia 768-28	F0QXN6	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Vulcanisaeta moutnovskia

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl acetate + H2O	-	Vulcanisaeta moutnovskia	4-nitrophenol + acetate	-	?
additional information	no activity with 4-nitrophenyl decanoate	Vulcanisaeta moutnovskia	?	-	?

Subunits

Subunits	Comment	Organism
homodimer	-	Vulcanisaeta moutnovskia

Synonyms

Synonyms	Comment	Organism
VmoLac	-	Vulcanisaeta moutnovskia

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	assay at	Vulcanisaeta moutnovskia

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
128	-	Tm-value	Vulcanisaeta moutnovskia

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0245	-	4-nitrophenyl acetate	pH 6.5, 50°C	Vulcanisaeta moutnovskia

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	assay at	Vulcanisaeta moutnovskia

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.00548	-	4-nitrophenyl acetate	pH 6.5, 50°C	Vulcanisaeta moutnovskia

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Release 2023.1 (January 2023)